Abstract: The WRKY protein family is the specific transcription factor family of plants, which is involved in plant growth, development and responses to various stresses. It is important to clarify the function of WRKY in biotic and abiotic stresses, and whether OsWRKY76 participates in regulating systemic acquired resistance in rice. Through analyzing the amino acid sequences, it was found that OsWRKY76 had a typical WRKY domain and belonged to the WRKY-GCM1 zinc finger WRKY superfamily. A 300 bp cDNA fragment of OsWRKY76, OsWRKY76-300, was cloned by PCR from the cDNAs of rice. A 500 bp fragment of GUS gene was amplified and was used as a linker. Both fragments were ligated into pENTR L16 vector, which was the entry vector in Gateway system, to form a hairpin vector in which two reverse repeated fragments of OsWRKY76-300 were linked by GUS 500 fragment. The inserted hairpin fragment was recombined into the inducible binary vector GVG-TA7002 and the constitutive binary vector Ubi-C1300, respectively, by LR reaction. And then the RNA interference vectors were obtained. Agrobacteria-mediated method was used to transform the inducible binary RNAi vector into rice. At last, seven transformed lines were got, and the expression level of OsWRKY76 was assayed by RT-qPCR. The expression of OsWRKY76 was silenced in two lines.

Key words: Rice, WRKY transcription factor, RNA interference, Vector construction, Expression analysis

CLC Number: 

• S511.03

• Q943.2