Abstract: Gliadin is a moiety of wheat prolamins, which have variety-specificity. The regulators and transcript genes of gliadins are regularly distributed on all the chromosomes of wheat. Therefore,gliadin was regarded as fingerprint of wheat variety. And the gliadins can be used to identify seed purity. The Jimai 31,Jinqiang 11,Jinqiang 13 were used as materials to study the method of purity identification in the different seed development stage. In this paper, the expression pattern of gliadin was taken as the research object to make a clear distinction of the whole bands of gliadin after 5, 10, 15, 20, 25, 30, 35 d, mature seed of flowering to study the earliest time point of seed quality testing by A-PAGE. The results showed that the expression of gliadin was not detected from the seed at 5-10 d after anthesis, and few bands of gliadins was detected from the seed at 15 d after anthesis, the complete bands was obtained from the seed at 20-25 d after anthesis, which was same as the mature seed. Therefore, the seed at 20 d after anthesis can be used to identify the seed purity. So, the purity of Jinqiang 11 wheat planted in the field 20-25 d after anthesis was identified to verify the feasibility and accuracy of the method. Our method can find the mixed seeds, and the average purity of Jinqiang 11 was 90.88%. The findings of this study provides a theoretical basis for finding the earliest development stage of immature grains for gliadin identification and purity identification before harvest, and provides a fast, simple and efficient identification technology to identify seed purity for seed enterprise.

Key words: Wheat, Gliadin, Expression of gliadin, Flowering tag, Seed quality testing

CLC Number: 

• S512.01

• Q512.5