Cloning, Expressing and Antibacterial Activity of Omiganan Antimicrobial Peptide

doi:10.7668/hbnxb.20191824

Abstract

Abstract: In order to obtain antimicrobial peptide Omiganan and research its antibacterial activity. In this research, the nucleotide sequence of Omiganan was obtained respectively by genetic engineering technology according to E. coli and Pichia pastoris codons preference and the primary structure of Omiganan antimicrobial peptide(NH₂-ILRWPWWPWRRK-COOH),following specific primers were designed to amplify Omiganan gene by PCR. And then the pET32a-Omiganan prokaryotic expression vector and pPIC9K-Omiganan Pichia pastoris expression vector were constructed, the Omiganan recombinant protein was expressed by IPTG 25℃ and IPTG 37℃ different temperature and self-induction expressing system in E.coli. The Omiganan antimicrobial peptide was expressed by 1% concentration methanol in Pichia pastoris.The results showed that the expression of Omiganan recombinant protein was the highest after induction for 14 h, while there was no significant difference between different IPTG temperature. Antibacterial experiment showed the Omiganan antimicrobial peptide Omiganan expressed by Pichia expression system had strong activity against E.coli and Staphylococcus aureus.

Key words: Omiganan, Antibacterial peptide, Cloning, Expressing, Antibacterial activity

CLC Number:

XIE Kun, LI Mijie, JIANG Chengyan, LU Haiju, KONG Qiong, HE Chao, SHEN Dengrong. Cloning, Expressing and Antibacterial Activity of Omiganan Antimicrobial Peptide[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 62-67. doi: 10.7668/hbnxb.20191824.

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http://www.hbnxb.net/EN/Y2021/V36/I2/62

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