Molecular Marker Detection and Utilization for HMW-GS 1By8 Gene in Wheat

doi:10.7668/hbnxb.2010.04.007

Abstract

Abstract: Wheat processing quality is closely correlated with composition and quantity of glutenin，in particular with the high-molecular weight glutenin subunits(HMW-GS) encoded by the Glu-1 locus. The specific PCR marker of 1By8 was applied to detect the presence of the HMW-glutenin 1By8 gene in 150 wheat varieties and lines used in Huanghuai Wheat Region. The results showed that the PCR marker could amplify a 527 bp fragment in 43 varieties (lines) and the positive control，Tasman，comprising the 1By8 gene，while other 107 varieties(lines) and the negative control(Kukri) did not show the specific product. The probability of the 1By8 gene was 28. 6% . The results corresponding to those by protein SDS-PAGE indicated that this marker was reliable and effective and could identify the presence of the HMW-glutenin 1By8 gene. Compared with SDS-PAGE detection，PCR is quicker ，e asier and accurate.

Key words: Wheat, High molecular weight glutelin, 1By8 subunit, Molecular marker detection, Utilization

CLC Number:

HE Sheng-lian, LEI Zhen-sheng, WU Zheng-qing, ZHAO Xian-lin, FANG Bao-ting, YANG Pan, YANG Hui-min, HE Ning, WANG Mei-fang, LI Wei. Molecular Marker Detection and Utilization for HMW-GS 1By8 Gene in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 35-39. doi: 10.7668/hbnxb.2010.04.007.

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