Analysis of Bacillus thuringiensis Strains Containing cry7 Gene

doi:10.7668/hbnxb.2010.04.008

Abstract

Abstract: Based on PCR amplification using universal primers，18 strains of Bt containing cry7 gene were obtained and analysis further about crystal shape，bioassay，toxin protein and gene type. Bi-pyramidal and irregular crystal shape were presented in these strains. Toxin from Bt strain appeared to contain a prominent protein band at a molecular weight of 130 kDa on SDS-PAGE. Genotypic investigations showed that these strains possessed the cry7 gene， and the amplified fragments were 1 300 bp. Bioassay results showed that the WZ-9 strain was only harmful to larvae of Henosepilachna vigintioctomaculata，c orrected mortality was 93. 1% at 72 h. And the strain WCG-10 showed weak toxicity，corrected mortality was 44. 8% at 72 h. Other strains were no toxicity against this insect. The full length toxin gene，cry7Ab3，was amplified and submitted to the committee of Bt toxin nomenclature (GenBank Number: BI1015188).

Key words: Bacillus thuringiensis, cry7 gene, Henosepilachna vigintioctomaculata

CLC Number:

SONG Ping, GUO Li-wei, SU Jun-ping, WANG Qin-ying, WANG Hui. Analysis of Bacillus thuringiensis Strains Containing cry7 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 40-43. doi: 10.7668/hbnxb.2010.04.008.

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