Construction of Expression Vector with Glyphasate-resistant Gene and Its Genetic Transform in Maize

doi:10.7668/hbnxb.2010.04.009

Abstract

Abstract: High efficiency binary expression vector was constructed based on pCambia3301 vector，modified by the components of maize ubiquitin promoter，transit peptide of Arabidopsis thaliana chlorophyll (Ctp) gene，and resistance to glyphosate Epsps gene. The recombinant plasmid was transferred into Agrobacterium tumefaciens LBA4404 by freeze-thawing method，then transformed into maize immature embryos. The plants resistant to glyphosate with non-antibiotic marker gene were obtained. The preliminary identification in the field demonstrated that the transgenic maize plants were capable of resisting glyphosate to some extent.

Key words: Glyphosate, Epsps, Expression vector, Maize, Genetic transformation

CLC Number:

Q786

LI Jing-na, LIU Ya, LI Xiang, YUAN Qian-hua, ZHAO Jiu-ran. Construction of Expression Vector with Glyphasate-resistant Gene and Its Genetic Transform in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 44-48. doi: 10.7668/hbnxb.2010.04.009.

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References

[1] 赵久然,王荣焕,史洁慧,等.国内外玉米动态及展望[J].作物杂志,2008(5):5-9.

[2] Baylis A D.Why glyphosate is a global herbicide:Strengths,weednesses and prospects[J].Pest Manag Sci,2000,56:299-308.

[3] Christensen A H,Sharrok R A,Quail P H.Maize polyubiquitin genes:structure,thermal perturbaticn of expression and transcript splicing and promoter activity following transfer to protoplasts electroporation[J].Plant Mol Biol,1992,18:675-689.

[4] Gordon-kamm W J,Spencer T M,Mangano M L,et al.Transformationof maize cells and regeneration of fertile transgenic plants[J].Plant Cell,1990,2:603-618.

[5] Ishida Y,Saito H,Ohta S,et al.High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens[J].Nat Biotechnol,1996,4:745-750.

[6] Frame B R,Shou H,Chikwamba R K,et al.Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system[J].Plant Physiol,2002,129(1):13-22.

[7] Frame B R,McMurray J M,Fonger T M,et al.Improved Agrobacte-rium-mediated transformation of three maize inbred lines using MS salts[J].Plant Cell Rep,2006,25(10):1024-1034.

[8] Fromm M E,Taylor L P,Walbot V.Stable transformation of maize after transfer by electroporation[J].Nature,1986,319(27):791-793.

[9] 刘小红,张红梅,张红伟,等.抗除草剂转基因玉米研究[J].西南农业学报,2007,20 (1):53-57.

[10] 朱常香,宋云枝,张杰道,等.抗虫、抗除草剂转基因玉米的获得及遗传研究[J].山东农业大学学报,2002,33 (2):120-125.

[11] 王景雪,赵福永,徐培林,等.油菜转抗草甘膦、抗虫基因获得双抗植株[J].遗传学报,2005,32 (12):1293-1300.

[12] Spencer T M,Gordon-Kamm W J,Daines R J,et al.Bialaphos selection of stable transformants from maize cell culture[J].Theor Appl Genet,1990,79(5):625-631.

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