Attainment and Identification of Transgene Rape (Brassica napus)with Bt and Antibiotic Peptide Syncretic Gene

doi:10.7668/hbnxb.2011.02.011

Abstract

Abstract: Rape is one of important oil crop that wei'e widely grown in China.The diseases occures to rape easily, especially for white spot and black spot that caused by fungi.The variety of Huayou No.6 was used for tranferring Bt and Antibiotic peptide syncretic gene(Bt-AMP),glyphosate as selective marker,by the method of agrobacterium-modiated transformation.The test of PCR and southern blot proved the exogenous DNA was integrated on the genome of rape;RT-PCR test showed the expression of transcriptional level of the introduced gene in rape plant.The tranformation rate of Bt-AMP Was 2.33%.The research provided new germ for rape breeding of disease resistance.

Key words: Rape, Antibiotic peptide, Bt, Syncretic gene, Transformation

CLC Number:

S55.4

YANG Kai, HAN Wei, WEN Ying, LIU Dan-dan, XUE Chun-lei, LU Xiao-ping. Attainment and Identification of Transgene Rape (Brassica napus)with Bt and Antibiotic Peptide Syncretic Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 49-54. doi: 10.7668/hbnxb.2011.02.011.

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