Molecular Cloning and Sequence Analysis of Porcine Gene Reg3

doi:10.7668/hbnxb.2011.02.012

Abstract

Abstract: In order to clone and analyze the gene Re93 in intestinal canal tissues of pig,the total RNA was extracted and mRNA sequence of Re93 gene was amplified by RT-PCR method which used two designed primers.The PCR productswere ligated into the pMD-19T vector,and then transformed into competent cells of E.coli JMl09.The sequence was analyzed to identify the recombinant plasmid.The identification analysis showed that the Re93 nucleotide sequence shared 83.7%,82.3%and 82 2% homology with that of human,sheep and horse respectively,revealing porcine Re93 gene had been successfully cloned in the present study.The sequence has been submitted to Gen-Bank(Accession FJ53 1494).

Key words: Pig, Reg3 gene, Molecular cloning, Sequence analysis

CLC Number:

Q785

LI Wen-lin, CHEN Fu-wei, WANG Wei-jie, HAN Li-qiang, WANG Yue-ying, LI Hong-ji, LIU Hong-liang, YANG Guo-yu. Molecular Cloning and Sequence Analysis of Porcine Gene Reg3[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 55-57. doi: 10.7668/hbnxb.2011.02.012.

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