Cloning and Response to Environmental Cues of BnCOP9 from Brassica napus

doi:10.3969/j.issn.1000-7091.2012.03.002

Abstract

Abstract: Plant COP9(Constitutively photomorphogenic 9)signalosome(CSN)is a mufti-subunit complex in?which CSN8 is an obligatory subunit. To clone CSN8 and investigate its response to environmental cues in oilseed?rape，a full length cDNA of Brassica napus CSN8 ( BnCOP9)was isolated from the line Zhongshuang9 by homology?cloning approach. Sequence analysis showed that BnCOP9 contains a PCI domain in the central region and 197 amino acids exhibiting a 92% identity with Arabidopsis CSN8(AtCOP9).Quantitative RT-PCR analysis showed that?BnCOP9 expression were promptly activated by Sclerotinia sclerotiorum，wound treatment and MeJA，while suppressed by Benzothiadiazole，suggesting that BnCOP9 is in response to environmental stresses and play a role in?crossing-talk of SA-and JA-mediated signaling and the molecular network of oilseed rape response against both S.?sclerotioru and wound. These results provide a cue to study a role of the CSN complex in defense responses in oilseed rape.?

Key words: Brassica napus , BnCOP9, Gene cloning, Induced expression

CLC Number:

S565.4
Q78

WANG Zheng, TAN Xiao-li, ZHANG Zhi-yan, GU Shou-lai, LI Guan-ying, CHEN Yu. Cloning and Response to Environmental Cues of BnCOP9 from Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 6-11. doi: 10.3969/j.issn.1000-7091.2012.03.002.

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