Abstract: In order to identify the genetic characteristics of the H-FABP gene, DNA pooling and direct sequencing technique were used for rapid screening of H-FABP gene polymorphism, the physicochemical properties of the encoded amino acids and the protein structure and function were predicted by bioinformatics in Gansu black pig. The result showed that 4 mutation sites were found in the region of the H-FABP gene amplification in the Gansu black pig. C⁷³⁴T-Intron1, T¹⁵²C-Exon2, G³⁰A-Intron2, T¹²¹G-Intron2, respectively, it was shown that the allele frequencies were significant differences before and after mutation;the CDS region of H-FABP gene in Gansu black pig was 402 bp, encoding 133 amino residues, and T¹⁵²C were missense mutations, was found in CDS region. Amino acids were converted from threonine (Ile)to threonine (Thr). The H-FABP gene encoding protein of black pig was a stable hydrophilic protein, protein secondary structure and tertiary structure were mixed type, and the proportion of beta folding was the highest. The results of protein function prediction showed that there were 19 potential phosphorylation and 1 N-glycosylation sites, it might affect the increase of fat content in the H-FABP gene regulation muscle;Homology comparison and phylogenetic tree analysis showed that it had most similar genetic relationship between Artiodactyl and Gansu black pig. These provided a theoretical basis for the molecular genetic markers of the intramuscular fat content deposition in Gansu black pigs, the polymorphism screening of the H-FABP gene, it has become a hot spot of molecular research in the improvement of meat quality traits in domestic animals.

Key words: Gansu black pig, H-FABP gene, DNA pooling, Single nucleotide polymorphisms, Bioinformatics

CLC Number: 

• S828

• Q78