Expressing of Fusion Gene of SeNHX1 and GFP and Its Tolerance to Salt in Yeast

doi:10.7668/hbnxb.2008.04.014

Abstract

Abstract: In order to study effect of the gene SeNHX1 on yeast tolerance to salt, vector pYES2 - SeNHX1 -GFP、 pYES2 - SeNHX1 and pYES2 - GFP was constructed and transformed into yeast W303.Transformed W303 was cultured in CM -ura medium with D -gal which induced the vector expressing in Yeast1Bright green under fluorescence microscopewas observed in W303( pYES2 -SeNHX1 -GFP) and W303( pYES2 - GFP), but not in W303( pYES2 -SeNHX1).W303( pYES2 - SeNHX1 -GFP) and W303( pYES2 - SeNHX1) grew more slowly than W303( pYES2 - GFP) in 0- 48 h, it grew fast after 48 h1It indicated that the vector expressed correctly in yeast and gene SeNHX1 could enhance the tolerance to salt.

Key words: SeNHX1 gene, GFP gene, Yeast, Vector, Growth curve, Fluorescence, Salt tolerance

CLC Number:

Q786

YANG Xiao-ling, JI Jing, WANG Gang, YANG Shao-hui, SUN Jian-xue, ZHANG Da-wei. Expressing of Fusion Gene of SeNHX1 and GFP and Its Tolerance to Salt in Yeast[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 60-64. doi: 10.7668/hbnxb.2008.04.014.

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