Study on Isolation and Culture of Intestinal Epithelial Cell in Chicken Embryo

doi:10.7668/hbnxb.2014.05.015

Abstract

Abstract: This current study was conducted to investigate the biological characteristics of chicken IECs(Intestinal epithelial stem cells)by isolating and culturing in vitro.IECs were isolated from small intestine of 18 day-old chicken embryos.The suitable culture medium for IECs was DMEM/F12.Then its proliferation was analyzed by subculture and the growth curve.The purified chicken intestinal epithelial stem cells were identified by morphology,immunohistochemical methods and RT-PCR.The results showed that chicken IECs can be cultured in vitro and have been passed to 11 generation.The purified IECs were positive by morphology,immunohistochemical methods and RT-PCR.To sum up,IECs can be isolated from small intestine,which can proliferate rapidly in vitro.

Key words: Chicken, IECs, Isolation and culture in vitro, Identification

CLC Number:

S831

PEI Pei, XIONG Hui, CUI Peng, GUAN Wei-jun, MA Yue-hui. Study on Isolation and Culture of Intestinal Epithelial Cell in Chicken Embryo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 85-91. doi: 10.7668/hbnxb.2014.05.015.

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http://www.hbnxb.net/EN/Y2014/V29/I5/85

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