Regulating Effects of Exogenous Spermidine on Anaerobic Respiratory Metabolism in the Roots of Maize Seedling under Water-logging Stress

doi:10.7668/hbnxb.2015.04.020

Abstract

Abstract: In order to elucidate the mechanism that polyamines improve the resistance of maize seeding to water-logging,two maize cultivars,Zhengdan No.958 (ZD 958:water-logging-sensitive)and Xundan No.22 (XD 22:water-logging-tolerant),were used as experiment materials,three treatments were implemented with control(CK),water-logging(WL) and WL+Spd (1 mmol/L).On the 0,2nd,4th,6th of treatment,the activities of anaerobic respiration enzymes (pyruvate decarboxylase:PDC,lactic dehydrogenase:LDH and alcohol dehydrogenase:ADH) and the contents of metabolite (ethanol and lactate) and Spd were detected in maize seedling roots,and the relative drought matter increase rate (RDMIR) of maize seedlings was also calculated.The regulating effects of exogenous spermidine(Spd) on the anaerobic respiration metabolism in maize seedling roots were studied under water-logging stress.The results showed the activities of anaerobic respiration enzymes,the contents of metabolite and Spd in two maize seedling roots had no obvious changes under normal growth conditions,and there was also no obvious difference between two cultivars;however,under water-logging stress,these enzyme activities,ethanol,lactate and Spd contents in two maize seedling roots increased,and the increases in ADH activity,ethanol and Spd contents in XD 22 was more significant than those in ZD 958,but the increases in LDH activity and lactate content in ZD 958 was more significant than that in XD 22;after treatment with Spd,the activity of ADH,ethanol and Spd contents increased and the LDH activity and lactate content decreased in two maize seedling root,the changes in these indexes in ZD 958 were more significant than those in XD 22.The results suggest that there are close relationships between polyamines and anaerobic respiration metabolism in maize seedling root under water-logging stress.On the one hand,Spd could decrease the injury of acetaldehyde on plant cell via improving the activity of ADH and increasing ethanol and Spd contents in maize seedlings roots,on the other hand,Spd could inhibit excessive lactate to avoid excessive acidification of cytoplasm via reducing LDH activity,relieve the injury of water-logging on maize seedling,and promote drought matter accumulation and the growth of maize seedling.

Key words: Spermidine, Water-logging stress, Maize, Anaerobic respiration

CLC Number:

S513
Q945.79

DU Hong-yang, CHENG Ming-ming, YANG Qing-hua, LIU Huai-pan. Regulating Effects of Exogenous Spermidine on Anaerobic Respiratory Metabolism in the Roots of Maize Seedling under Water-logging Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 110-116. doi: 10.7668/hbnxb.2015.04.020.

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References

[1] 葛均筑,展茗,赵明,等.渍涝胁迫对玉米生理生化的影响研究进展[J].中国农学通报,2012,28(21):7-11.
[2] 汪天,王素平,郭世荣,等.外源亚精胺对根际低氧胁迫下黄瓜幼苗光合作用的影响[J].应用生态学报,2006,17(9):1609-1612.
[3] 刘盼盼,伍大利,刘畅,等.淹涝和氮形态对苗期玉米伤流液流量及组分的影响[J].华北农学报,2013,28(3):133-138.
[4] Christianson J A,Llewellyn D J,Dennis E S, et al. Global gene expression responses to waterlogging in roots and leaves of cotton (Gossypium hirsutum L.) [J].Plant and Cell Physiology,2010,51:21-37.
[5] 任佰朝,张吉旺,李霞,等.大田淹水对夏玉米叶片衰老特性的影响[J].应用生态学报,2014,25(4):1022-1028.
[6] Jackson M B,Davies W J,Else M A.Pressure-flow relationships,xylem solutes and root hydraulic conductance in flooded tomato plants [J].Annals of Botany,1996,77:17-24.
[7] Drew M C.Oxygen deficiency and root metabolism:Injury and acclimation under hypoxia and anoxia[J].Annual Review of Plant Physiology and Plant Molecular Biology,1997,48:223-250.
[8] Bansal R,Srivastava J P.Antioxidative defense system in pigeonpea roots under waterlogging stress[J].Acta Physiologiae Plantarum,2012,34:515-522.
[9] 胡晓辉,郭世荣,李璟,等.低氧胁迫对黄瓜幼苗根系无氧呼吸酶和抗氧化酶活性的影响[J].武汉植物学研究,2005,23(4):337-341.
[10] Jia Y,Sun J,Guo S R, et al. Effect of root-applied spermidine on growth and respiratory metabolism in roots of cucumber (Cucumis sativus) seedlings under hypoxia1[J].Russian Journal of Plant Physiology,2010,57(5):648-655.
[11] Bai T,Li C Y,Ma F W,et al.Exogenous salicylic acid alleviates growth inhibitionand oxidative stress induced by hypoxia stress in malus robusta read[J].Journal of Plant Growth Regulation,2009,28(4):358-366.
[12] 李程,裴忠孝,甘林叶,等.光周期对春石斛开花及多胺含量的影响[J].植物生理学报,2014,50(8):1167-1170.
[13] 杜红阳,刘怀攀,李潮海,等.植物体内特殊形态多胺与水分胁迫关系研究进展[J].河南农业科学,2007(12):9-12.
[14] Gupta K,Dey A,Gupta B.Plant polyamines in abiotic stress responses[J].Acta Physiologiae Plantarum,2013,35:2015-2036.
[15] Liu H P,Dong B H,Zhang Y Y,et al.Relationship between osmotic stress and the levels of free,conjugated and bound polyamines in leaves of wheat seedlings [J].Plant Science,2004,166(5):l261-1267.
[16] Slathia S,Sharma A,Sikander C P.Influence of exogenously applied epibrassinolide and putrescine on protein content antioxidant enzymes and lipid peroxidation in lycopersicon esculentum under salinity stress[J].American Journal of Plant Science,2012,3(6):714-720.
[17] Barnawal D,Bharti N,Maji D,et al.1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing rhizobacteria protect Ocimum sanctum plants during waterlogging stress via reduced ethylene generation[J].Plant Physiology and Biochemistry,2012,58(9):227-235.
[18] 程明明,杜红阳,刘怀攀.外源亚精胺对涝胁迫下玉米幼苗根和氧化伤害缓解效应[J].南方农业学学报,2015,46(1):36-41.
[19] Du H Y,Zhou X G,Yang Q H,et al.Changes in H⁺-ATPase activity and conjugated polyamine contents in plasma membrane purified from developing wheat embryos under short-time drought stress[J].Plant Growth Regulation,2015,75(1):1-10.
[20] Gong M,Li Y J,Chen S Z.Abscisic acid-induced thermotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems[J].Journal of Plant Physiology,1998,153(4):488-496.
[21] Mustroph A,Albrecht G.Tolerance of crop plants to oxygen deficiency stress:Fermentative activity and photosynthetic capacity of entire seedlings under hypoxia and anoxia [J].Physiologiae Plantarum,2003,117:508-520.
[22] Waters I,Morell S,Greenway H,et al.Effects of anoxia on wheat seedlings.Ⅱ.Influence of O₂ supply prior to anoxia on tolerance to anoxia,alcoholic fermentation,and sugar levels [J].Journal of Experimental Botany,1991,42:1437-1447.
[23] Bergmeger H U.Merthods of enzymatic analysis[M].3rd.Verlag Chemse:Weinhein Press,1983:118-125.
[24] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J].Analytical Biochemistry,1976,72:248-254.
[25] Kato-Noguchi H.Evaluation of the importance of lactate for the activation of ethanolic fermentation in lettuce roots in anoxia[J].Physiologiae Plantarum,2000,109:28-33.
[26] Bergmeger H U.Merthods of enzymatic analysis[M].3rd.Verlag Chemse:Weinhenin Press,1983:598-616.
[27] Kiriakos K,Maria D,Christakis H,et al.A narrow-pore HPLC method for the identification and quantitation of free,conjugated,and bound polyamines [J].Analytical Biochemistry,1993,214:484-489.
[28] Trought M C T,Drew M C.Effects of waterlogging on young wheat plants (Triticum aestivum L.) and on soil solutes at different temperatures[J].Plant and Soil,1982,69:311-326.
[29] Roberts J K,Callis J,Jardetzky O,et al.Cytoplasmic acidosis as a determinant of flooding in tolerance in plants[J].Proceedings of the National Academy of Sciences of the United States of America,1984,81:6029-6033.
[30] Ismond K P,Dolferus R,De-pauw M,et al.Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway[J].Plant Physiology,2003,132:1292-1302.
[31] Armstrong W,Br ndle R,Jackson M B.Mechanisms of flood tolerance in plants[J].Acta Botanica Neerlandica,1994,43:307-358.
[32] Guo S R,Nada K,Katoh H,et al.Differences between tomato(Lycopersicon esculentum Mill.) and cucumber (Cucumis sativus L.) in ethanol,lactate and malate metabolisms and cell sap pH of roots under hypoxia[J].Journal of the Japanese Society for Horticultural Science,1999,68(1):152-159.
[33] 康云艳,郭世荣,段九菊,等.24-表油菜素内酯对低氧胁迫下黄瓜根系抗氧化系统及无氧呼吸酶活性的影响[J].植物生理与分子生物学学报,2006,32(5):535-542.
[34] 徐迎仓,王静,刘华,等.外源精胺对小麦幼苗抗氧化酶活性的促进作用[J].植物生理学报,2001,27(4):349-352.

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