Quantitative Trait Loci Analysis of Flag Leaf Size and Grain Relative Traits in Winter Wheat

doi:10.7668/hbnxb.2018.05.001

Abstract

Abstract: To identify quantitative trait loci (QTL) for flag leaf length (FLL),width (FLW),area (FLA),thousand grain weight (TGW),grain number per spike (GNS),grain weight per spike(GWS) and understand the genetic relationship among those traits,we developed a doubled haploid(DH)population with 216 lines, derived from the cross between Nongda 3338 and Jingdong 6. The field experiments were carried out in Linfen and Yuncheng in 2015 and 2016, and QTL analysis was conducted by composite interval mapping method combining with genetic maps containing 469 markers. The results showed that a total of 105 additive QTLs were detected,forty of them were QTLs for flag leaf traits and sixty-five were for grain traits,with single QTL explaining 2.28%-39.91% of the phenotypic variances. Several QTLs were identified in the same region or adjacent regions on the 1B,2A,2B,2D,3A,4A,4B,4D,6A,6D and 7A chromosome, these QTLs were closely linked to each other or multi-effect, confirmed those phenotypic data were closely-related under QTL level. Seven QTLs (Qflw-4A.1, Qflw-4B.1, Qflw-4D.1, Qfla-4B, Qtgw-4A.1, Qtgw-4B.2, Qtgw-4B.3) had high LOD value were detected repeatedly under three or four environments. Those seven stable expression QTLs might have great significance for MAS breeding and gene cloning program.

Key words: Wheat, Flag leaf traits, Grain relative traits, Genetic correlation, QTL analysis

CLC Number:

S512.03

LU Lahu, YANG Bin, ZHANG Ting, ZHANG Wei, YUAN Kai, SHI Xiaofang, PENG Huiru, NI Zhongfu, SUN Qixin. Quantitative Trait Loci Analysis of Flag Leaf Size and Grain Relative Traits in Winter Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 1-8. doi: 10.7668/hbnxb.2018.05.001.

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