Analyzing Polymorphism of SLA-DQA Gene exon4

doi:10.3969/j.issn.1000-7091.2012.03.017

Abstract

Abstract: The polymorphisms of the fourth exon of SLA-DQA gene were detected by PCR-SSCP，aiming to get?molecular genetics information about SLA-DQA gene. The results showed that there were 4 alleles and 6 genotypes in?Landrace，Large white and Duroc breeds (210 individuals). 6 genotypes(A，AB，CC，CD，BB，BC)was detected in?Duroc，AA，AB，CC in Large white，AA，AB，CC，CD，BB in Landrace. alleles A was the predominant allele，AA was?the predominant genotype in three breeds. There are 3 mutations in exon 4，(A→G) at 5126bp，(A→T)at 5158bp?and(G→A)at 5233bp respectively. (A→G)mutation leads to the changes of encoding amino acid，I→V on protein?level. This polymorphic locus in three breeds is deviated from Hardy-Weinberg equilibrium by χ2test(P ＜0. 05).?The polymorphism information content of Landrace and Large white are 0. 3233 and 0. 4548. respectively indicates?moderate polymorphism(0. 25 ＜ PIC ＜0. 5)，Duroc is0. 6101 indicates highly polymorphism(PIC ＞0. 5)，the genotypes are significant among three breeds by χ2test(P ＜ 0. 01). It showed that the exon4 of SLA-DQA gene in three?breeds had a high level of polymorphism.

Key words: DQA gene, P olymorphism, PCR-SSCP, Allele

CLC Number:

S828

KONG Jing-jing, GUN Shuang-bao, YANG Qiao-li, WANG Guo-wang, LIU Li-xia, LI Feng-song, JIANG Tian-tuan, FENG Yang, ZHAO Sheng-guo. Analyzing Polymorphism of SLA-DQA Gene exon4[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 86-90. doi: 10.3969/j.issn.1000-7091.2012.03.017.

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