Polymorphism of BoLA-DRA*Exon2-intron2 in Yak and Bovine

doi:10.7668/hbnxb.2015.04.013

Abstract

Abstract: DRA gene,coding α chain of Ⅱ antigen molecules,belongs to the family of BoLA Ⅱ genes.The antigen-binding region coded by exon2 of DRA gene plays an important role in antigen presentation.This study detected the genetic polymorphisms in exon2 and intron2 of BoLA-DRA gene in Tianzhu White yak,Datong yak,Gannan yak and cattle by PCR single-strand conformational polymorphism (SSCP).The results showed that two SNPs,T >C at 197th and G >A at 116th,corresponding to alleles A,B and C were detected,and the polymorphism was moderate level with PIC in the range of 0.249-0.424 in the exon2 of BoLA-DRA.Four mutations,including one T >C,one C >T and one A >G,corresponding to alleles a-d were identified,and moderate or high polymorphism with PIC in the range of 0.389-0.515 was observed in the intron2 of BoLA-DRA in yak and cattle.Deduced nucleotide sequences of BoLA-DRA^* exon2 between yak,bovine and goat were the highest on homology and the phylogenetic distance consistent with their genetic relationship.

Key words: Yak, Bovine, DRA gene, PCR-SSCP, Polymorphism

CLC Number:

S823

LI Yan-qing, NIU Xiao-liang, HU Jiang, YAN Wei, LUO Yu-zhu, WANG Wen-biao, CAIRANG Nao-ri. Polymorphism of BoLA-DRA^*Exon2-intron2 in Yak and Bovine[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 72-77. doi: 10.7668/hbnxb.2015.04.013.

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http://www.hbnxb.net/EN/Y2015/V30/I4/72

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