Effects of Ghrelin on in vitro Development of Mouse Embryos and the Expression of Ghsr-1a in Different Embryos

doi:10.7668/hbnxb.2015.04.014

Abstract

Abstract: The objective of the present study was to investigate the effect of addition of ghrelin to in vitro culture medium on preimplatation development quality of different sources(in vivo, in vitro fertilization,parthenogenetic) mouse embryos.The receptor of Ghrelin, Ghsr-1a which mRNA and protein expression also been checked in preimplatation embryos.The results showed that blastocyst rate of in vivo embryos was significantly decreased when 0.1 ng/mL Ghrelin added into in vitro culture medium (P <0.05).Blastocyst rate of in vitro fertilization embryos cultured in KSOM supplemented with 1 ng/mL ghrelin was significantly decline than control group (P <0.05).The mRNA levels and protein expression of Ghsr-1a were observed at different early stage of mouse.The transcription level of Ghsr-1a of 4-cell embryo was signification higher than other stages of embryos in in vivo embryos(P <0.05). Ghsr-1a levels was decreased from 8-cell to blastocyst in in vitro fertilization embryos,The mRNA levels of Ghsr-1a was declined from 2-cell to 8-cell and increased from 8-cell to blastocyt in parthenogenetic embryos and protein expression contrary.The protein expression of Ghsr-1a were decreased in blastocyst stage and same in vivo and in vitro fertilization embryos.Thus,ghrelin may inhibit embryo development quality through its receptors Ghsr-1a and give a reference for further researchers in function of ghrelin in mouse embryos.

Key words: Ghrelin, GHSR-1a, Mouse, Embryos

CLC Number:

S814.8

LI Yun-sheng, YANG Hong-xing, LIU Xiao-rui, XI Hai-tao, FANG Fu-gui, LIU Ya. Effects of Ghrelin on in vitro Development of Mouse Embryos and the Expression of Ghsr-1a in Different Embryos[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 78-83. doi: 10.7668/hbnxb.2015.04.014.

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References

[1] Gualillo O,Lago F,Gómez-Reino J,et al.Ghrelin,a widespread hormone:insights into molecular and cellular regulation of its expression and mechanism of action[J].FEBS Letters,2003,552(2/3):105-109.
[2] Horvath T L,Diano S,Sotonyi P,et al.Minireview:ghrelin and the regulation of energy balance-a hypothalamic perspective[J].Endocrinology,2001,142(10):4163-4169.
[3] Garcia M C,Lopez M,Alvarez C V,et al.Role of ghrelin in reproduction[J].Reproduction,2007,133(3):531-540.
[4] Harrison J L,Adam C L,Brown Y A,et al.An immunohistochemical study of the localization and developmental expression of ghrelin and its functional receptor in the ovine placenta[J].Reproductive Biology and Endocrinology,2007,5:25.
[5] Kawamura K,Sato N,Fukuda J,et al.Ghrelin inhibits the development of mouse preimplantation embryos in vitro [J].Endocrinology,2003,144(6):2623-2633.
[6] Tena-Sempere M.Ghrelin and reproduction:ghrelin as novel regulator of the gonadotropic axis[J].Vitamins and Hormones,2008,77:285-300.
[7] Dupont J,Maillard V,Coyral-Castel S,et al.Ghrelin in female and male reproduction[J].International Journal of Peptides,2010:158102.
[8] Fernández-Fernández R,Tena-Sempere M,Navarro V M,et al.Effects of ghrelin upon gonadotropin-releasing hormone and gonadotropin secretion in adult female rats: in vivo and in vitro studies[J].Neuroendocrinology,2005,82(5/6):245-255.
[9] Fernández-Fernández R,Navarro V M,Barreiro M L,et al.Effects of chronic hyperghrelinemia on puberty onset and pregnancy outcome in the rat[J].Endocrinology,2005,146(7):3018-3025.
[10] Li L,Ferin M,Sauer M V,et al.Serum and follicular fluid ghrelin levels negatively reflect human oocyte quality and in vitro embryo development[J].Fertility and Sterility,2011,96(5):1116-1120.
[11] Dovolou E,Messinis I E,Periquesta E,et al.Ghrelin accelerates in vitro maturation of bovine oocytes[J].Reproduction in Domestic Animals,2014,49(4):665-672.
[12] 谢体三,农微,张新民,等.Ghrelin对水牛体外受精和孤雌激活胚胎体外发育的影响[J].中国兽医学报,2009,29(11):1478-1480.
[13] Wang Z,Lin P,Yu S.Effects of ghrelin on developmental competence and gene expression of in vitro fertilized ovine embryos[J].Theriogenology,2013,79(4):695-701.
[14] Zhang K,Wei H X,Zhang Y H,et al.Effects of ghrelin on in vitro development of porcine in vitro fertilized and parthenogenetic embryos[J].The Journal of Reproduction and Development,2007,53(3):647-653.
[15] Du C,Li H,Cao G,et al.Expression of the orexigenic peptide ghrelin and the type 1a growth hormone secretagogue receptor in sheep oocytes and pre-implantation embryos produced in vitro [J].Reproduction in Domestic Animals,2010,45(1):92-98.
[16] Summers M C,Bhatnagar P R,Lawitts J A,et al.Fertilization in vitro of mouse ova from inbred and outbred strains:complete preimplantation embryo development in glucose-supplemented KSOM[J].Biology of Reproduction,1995,53(2):431-437.
[17] Quinn P,Kerin J F,Warnes G M.Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid[J].Fertility and Sterility,1985,44(4):493-498.
[18] Popelkova M,Sirotkin A V,Bezakova A,et al.Effect of IGF-I,leptin,ghrelin and MAPK-ERK on the nuclear maturation of bovine oocytes[J].Bulletin of the Veterinary Institute in Pulawy,2006,50(2):179-181.
[19] Dovolou E,Periquesta E,Messinis I E,et al.Daily supplementation with ghrelin improves in vitro bovine blastocysts formation rate and alters gene expression related to embryo quality[J].Theriogenology,2014,81(4):565-571.
[20] Mckee K K,Palyha O C,Feighner S D,et al.Molecular analysis of rat pituitary and hypothalamic growth hormone secretagogue receptors[J].Molecular Endocrinology,1997,11(4):415-423.

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