Influence of Activated Charcoal on Isolated Microspore Culture in Chinese Cabbage

doi:10.7668/hbnxb.2008.05.020

Abstract

Abstract: Fluency of activated charcoal on isolated microspore culture in Chinese cabbage was studied.The results showed that NLN-13 liquid medium with activated charcoal(0.5 g/L)was obviously better in frequency of microspore-derived embryos and frequency of regenerated plants.Frequency of microspore-derived embryos in N4 and N8 was increased 19 and 66.5 times.Isolated microspores from 24 varieties of Chinese cabbage were cultured in NLN-13 liquid medium with activated charcoal(0.5 g/L),20 varieties were produced microspore derived embryos.Embryo yield per bud(frequency microspore-derived embryos)was from 0.4 to 86.7.Microspore-derived embryos on B₅medium with 6-BA 0.2mg/L and NAA 0.02 mg/L and activated charcoal 0.5 g/L and 3% sucrose and 1%agar is good to regenerated plants.

Key words: Chinese cabbage, Isolated microspore culture, Microspore-derived embryos, Frequency, Activated charcoal

CLC Number:

S634.303.4

JIANG Wu-sheng, ZHANG Xiao-wei, YAO Qiu-ju, YUAN Yu-xiang, GENG Jian-feng. Influence of Activated Charcoal on Isolated Microspore Culture in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 93-96. doi: 10.7668/hbnxb.2008.05.020.

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http://www.hbnxb.net/EN/Y2008/V23/I5/93

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