SSR Fingerprinting and Genetic Distinctness of Pak-choi(Brassica rapa L.ssp.chinensis Makino)

doi:10.7668/hbnxb.2008.05.021

Abstract

Abstract: To develop DUS testing standards for Pak-choi,we collected and selected 80 Pak-choi varieties,which are landraces with different morphology and ecotype and hybrids in a large planting area in China.On the basis of botanic characters in vestigation,SSR fingerprinting and genetic distinctness of 80 non-heading Chinese cabbage varieties were an-alyzed by a set of SSR markers in this study.Out of SSR primer 131 pairs,20 pairs were screened out with polymorphism among 80 varities and 56 polymorphic bands were obtained.The polymorphism ratio was 73.68%,2.8 polymorphic bands in average and were amplified by a pair of SSR primers.80 varieties could be distinguished by a 5-primer-combination of Na12E02,Na12A08,Ni4D09,BRMS-269 and BRMS-296.SSR fingerprinting mapping for each variety was established.The results from genetic cluster showed that the varieties with similar morphological traits or the same origin have a close genetic base,87.50% of the tested varieties were distinguishable at a genetic distance threshold of 0.36.

Key words: Pakchoi, Simple sequence repeats (SSR), Fingerprinting

CLC Number:

S634.03

WANG Xiao-yi, YU Shuan-cang, ZHANG Feng-lan, YU Yang-jun, ZHAO Xiu-yun, ZHANG De-shuang. SSR Fingerprinting and Genetic Distinctness of Pak-choi(Brassica rapa L.ssp.chinensis Makino)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 97-103. doi: 10.7668/hbnxb.2008.05.021.

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