Abstract: Several factors that affected the ISSR-PCR applification ofO.racemosaTurcz.,such as concentration of template DNA,primer,Mg2+^{and dNTP were examined.The optimal ISSR-PCR reaction system in the experiment was established as follows:20 ng template DNA,0.24μmol/L primer,2.0 mmol/L MgCl_{2<.sub>and 0.15 mmol/L dNTPs.The research provided some foundamental data to the studies and use ofO.racemosaTurcz}}

Key words: Oxytropis racemosaTurcz., ISSR, PCR, Optimization

CLC Number: 

• Q346