Direct Amplification Minisatellite DNA Fingerprinting on Melon(Cucumis melo L)

doi:10.3321/j.issn:1000-7091.2006.03.018

Abstract

Abstract: The optimal reaction of DAMD(Direct Amplification of Minisatellite DNA by PCR) in melon was studied with a minisatellite primer YNZ22 and melon template DNA.The results showed that the optimum concentration of five important components i.e.Taq DNA polymerase,Mg²⁺,primer,template DNA and dNTPs in 20μL reaction system were 1U,2.5mmol/L,0.5mmol/L,30 ng,5mmol/L respectively.The minisatellite DNA fingerprinting of 28 melon cultivars were established by using optimum system with primer YNZ22.The primer could produce 13 bands,and 9 bands were polymorphic bands,the percentage of polymorphic bands(PPB) was 69%.The primer could identify 21 of 28 cultivars at once,its identification rate was 75%.

Key words: Cucumis melo, Minisatellite DNA, Fingerprinting map

CLC Number:

S652

WANG Zhang-jun, WANG Jian-she, LIU Ling, LIU Sheng-xiang, CHEN Su-sheng, XU Yong, WANG Yong-jian. Direct Amplification Minisatellite DNA Fingerprinting on Melon(Cucumis melo L)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 77-80. doi: 10.3321/j.issn:1000-7091.2006.03.018.

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