The Effect of Nicotinamide on Energy Metabolism of Mice Hepatocytes in vitro

doi:10.7668/hbnxb.2009.S1.029

Abstract

Abstract: To investigate the effect of nicotinamide (NA)on energy metabolism of mice hepatocytes in vitro,hepatocytes were cultured in the presence of 0,100 ,200, 300,400 and 500 μmol/L of NA for 24 h,then nitric oxide (NO), nitric oxide synthase (NOS)and three indexes lactic acid(LD), lactate dehydrogenase(LDH)and adenosine triphosphatase (ATPase) were assessed by kits.Results showed that 200 μmol/L instead of 500 μmol/L NA significantly stimulated energy metabolism of mice hepatocytes ,which suggested that NA can promote energy metabolism of mice hepatocytes in vitro.

Key words: Nicotinamide, Energymetabolism, Hepatocytes, Mouse

CLC Number:

S81.03

ZANGKun, LIUXiao-mu, SONGEn-liang, WANFa-chun, PANQing-jie. The Effect of Nicotinamide on Energy Metabolism of Mice Hepatocytes in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.029.

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http://www.hbnxb.net/EN/Y2009/V24/IS1/115

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