Primary Cloning of Mouse Prolactin-releasing Peptide cDNA

doi:10.7668/hbnxb.2007.06.041

Abstract

Abstract: To clone and analyse mouse prolactin-releasing peptide. Total RNA was extracted from hypothalamus and amplified by RT-PCR. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of E. coli DH5a, The positive cloning was sequenced and anelyzed.The cloned nucleotide sequence shared 86.3% hourology with the code zone sequence of rat prolactin-releasing peptide. The cloned nucleotide sequence was pert of mouse polactin-releasing peptide gene.

Key words: Mouse, PrRP, Cloning, Sequence

CLC Number:

ZHU He-shui, DU Jun-xia, WANG Xin-wei, WANG Yue-ying, WANG Yan-ling. Primary Cloning of Mouse Prolactin-releasing Peptide cDNA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 199-200. doi: 10.7668/hbnxb.2007.06.041.

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http://www.hbnxb.net/EN/Y2007/V22/I6/199

References

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