ACTA AGRICULTURAE BOREALI-SINICA ›› 2007, Vol. 22 ›› Issue (6): 195-198. doi: 10.7668/hbnxb.2007.06.040

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Study on Peroxidase, Superoxide Dismutase Activity and Soluble Protein Content Changes During Leaf Culture in vitro of Anthurium andraeanum

XIA Shi-yun1, MAI Yu-ling2, LIN Shu-han2, WU Guo-zhi3   

  1. 1. Tianjin Agricultural Biotechnology Research Center, Tianjin 300384, China;
    2. Shantou Zhongshu Flower Company Ltd, Shantou 515041, China;
    3. Tianjin Flower Garden, Tianjin 300112, China
  • Received:2007-01-31 Published:2007-12-28

Abstract: Changes in Peroxidase(POD), Superoxide Dismutase(SOD) activity and soluble protein content during the callus induction and buds differentiation in Anthuraum andraeanum lind were sagtudiedwith Guaiacol process, Nitroblue te-trazolium photoredudion method and Coomassie brilliant blue G-250 respectively. The results showed that POD, SOD and soluble protein content had a very high level in young leaves.However, there had a top level duringcallus induction stage. POD, SOD and soluble protein content start decrease; Activity of POD and SOD of "Arizon" had much higher than that of Pink Champion at the buds differentiation. After the plantlet transplanted, POD and SOD activity and soluble protein content kept low level in the two varieties since POD and SOD activity at this stage had still much higher than that of young leaves, soluble protein content had 1ower than that of young leaves.

Key words: Anthuraum andraeunum, In vitro, Peroxidase(POD), Superoxide dismutase(SOD) activity, Soluble protein concern

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Cite this article

XIA Shi-yun, MAI Yu-ling, LIN Shu-han, WU Guo-zhi. Study on Peroxidase, Superoxide Dismutase Activity and Soluble Protein Content Changes During Leaf Culture in vitro of Anthurium andraeanum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 195-198. doi: 10.7668/hbnxb.2007.06.040.

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