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Identification of RAPD-cultivar of Jujube

ZHIFu-jun1, JIAYan-li1, LIANGHai-yong2, LURui-jiang1, CHUFeng-jie1   

  1. 1. Shijiazhuang Pomology Institute, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050061, China;
    2. College of Forestry, Agricultural University of Hebei, Baoding 071001, China
  • Received:2009-03-17 Published:2009-12-31

Abstract: Five jujube total DNAs were extracted by Kit method and used for identification and cluster analysis was made based on RAPD method.12 primers were selected to generate stable and polymorphic DNA fragments.Total 34 RAPD fragments were amplified,20 of which were polymorphic.Each primper could generate 1 to 5 DNA fragments.The percentage of polymorphic loci was 58.82%.A dendrogram was established ,based on Hier archical cluster analysis which analyzed by DPS version software could divide five jujube cultivars into 2 groups,among which there were some genetic relations.

Key words: Jujube, RAPD, Cultivaridentification, Clusteranalysis

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Cite this article

ZHIFu-jun, JIAYan-li, LIANGHai-yong, LURui-jiang, CHUFeng-jie. Identification of RAPD-cultivar of Jujube[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.028.

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