Abstract: The pathogenic fungus of ring grain disease of Winter Jujubes was isolated,screened and identified, and its antagonistic bacterium was determined. Taking the fruits infected by ring grain disease of Winter Jujubes as the target,the pathogenic fungus of ring grain disease was confirmed by reinoculating test. The pathogenic fungus was identified by the morphological characteristics and ITS sequence analysis. Moreover,culturing characteristics, intracellular toxin and extracellular toxin was studied. The antagonistic bacterium was screened using the confront antibiotic culture experiment,and it was identified using the morphological,physiological and biochemical characteristics combined with 16S rDNA gene sequence analysis. The pathogenic fungus was successfully screened from the fruits infected by ring grain disease. Based on its morphology,cultural characteristics as well as ITS sequence analysis,the pathogenic fungus was identified as Botryosphaeria dothidea. The single factor experiments show that the optimal carbon sources,nitrogen sources,pH,growth temperature were sucrose,peptone,5,27 ℃,respectively. One bacterium isolated from the local soil showed strong antagonistic activity against the pathogenic fungus of ring grain disease,and it was identified as Bacillus sp.. The strain of Bacillus sp. has a potential application in the biological control to the ring grain disease of Winter Jujubes.

Key words: Winter Jujubes, Rind gain disease, Pathogenic fungus, Toxicity, Antagonistic bacterium

CLC Number: 

• S432.4