Abstract: In order to explore the preparation technology,safety and bacteriostatic effect of phosphorylated Agaricus blazei Murrill polysaccharide,a series of experiments were carried out.Single factor and orthogonal design method were used to observe the influences from phosphorylation reagent ratio,material ratio,time,temperature and pH value on the phosphate-group grafting amount of ABMP.In the safety evaluation test of phosphorylated ABMP,the acute toxicity test in mice was used to evaluate the safety.In the antibacterial test of the phosphoylated ABMP,the phosphoylated ABMP and ABMP were compared to verify the bacteriostatic effect on Streptococcus,Escherichia coli,Diplococcus and Salmonella bacteria.Results showed that the STPP/STMP ratio was 6:1,material ratio was 1:3,time was 5.5 h,temperature was 95℃,and pH value was 9,which was the optimal reaction condition.And the phosphate-group grafting amount at this moment was 9.14%.The acute toxicity test of phosphorylated ABMP showed that all indexes of mice were normal and safe.The results of antibacterial tests showed that the phosphoylated ABMP and ABMP had bacteriostatic effect on Salmonella bacteria and Escherichia coli,and the phosphorylated ABMP was better.Through the above three part tests,we obtained a good phosphorylation method and proved the safety of phosphorylated ABMP,and proved that the phosphorylated ABMP has a good bacteriostatic effect,which had further research value.

Key words: Phosphorylated Agaricus blazei Murrill Polysaccharide, Safety evaluation, Acute toxicity test, Antibacterial, Single factor test

CLC Number: 

• S432.4