Study on Antioxidant and Antibacterial Activity in vitro of Echinops latifolius Tausch Polysaccharides

doi:10.7668/hbnxb.2016.04.036

Abstract

Abstract: In this study antioxidant activity and antibacterial activity of Echinops latifolius Tausch polysaccharides was researched.The result showed that when the initial concentrations were same,ETCP has a highest effect of total reducing capacity,clearance of DPPH free radical in vitro,higher than ETPA,significantly higher than ETPB and ETPC.When the added concentrations were 0.1-0.3 mg/mL,ETCP has a little higher scavenging effects on free radicals than that of ETPA,but no obvious difference;when the added concentrations were 0.4 mg/mL,ETCP has a little higher scavenging effects on free radicals than that of ETPA,ETPA has a little higher scavenging effects than that of ETPB,and difference were not significant,they were significantly higher than that ETPC,when the added concentrations were 0.5 mg/mL,ETCP has a higher scavenging effects on free radicals than that of ETPA,ETPB,significantly higher than ETPC.Experimental condition,ETCP and ETPA were less sensitive to E.coli,the minimal inhibitory concentration(MIC) were 0.62 mg/mL, ETPB and ETPC were insensitive the MIC were 1.25 mg/mL; ETCP,ETPA,ETPB and ETPC were insensitive to Staphylococcus aureus,the MIC were 1.25,2.50,2.50,2.50 mg/mL respectively;ETCP,ETPB and ETPC were insensitive to Pseudomonas aeruginosa,the MIC were 5.00 mg/mL, ETPA had no inhibition to Pseudomonas aeruginosa;ETCP,ETPA,ETPB and ETPC were insensitive to Salmonella,the MIC were 2.50,2.50,1.25,5.00 mg/mL respectively.It means ETCP has the strongest antioxidant effect and secondly for ETPA,ETPB and ETPC were weak.ETCP and ETPA has a certain resistance to activity of E.coli, and ETPA,ETPB and ETPC has no antibacterial action to Staphylococcus aureus,Pseudomonas aeruginosa and Salmonella.

Key words: Echinops latifolius Tausch, Polysaccharide, Antioxidant activity, Antibacterial activity

CLC Number:

S853.74

YANG Bin, SA Ruli, ZHANG Yuemei, DALAI Baolige, SONG Yue, SONG Aijun, CHEN Wei, LU Yan, LIU Min, YANG Ying, ZHAO Shihua. Study on Antioxidant and Antibacterial Activity in vitro of Echinops latifolius Tausch Polysaccharides[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 233-238. doi: 10.7668/hbnxb.2016.04.036.

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References

[1] Abera B.Medicinal plant used in traditional medicine in Jimma zone,Southwest Ethiopia[J].Health Sci,2003,13:85-94.
[2] Oudhia P.Traditional medicinal knowledge about fireflies,photurissp[J].Insect Environment,2003,8(1):25.
[3] Shukla Y N.Chemical,botanical and pharmacological studies on the genus Echinops:a review[J].Journal of Medicinal and Aromatic Plant Sciences,2003,25:720-732.
[4] 周容,许晶晶,波拉提·马卡比力,等.新疆蓝刺头特征化学成分初步研究[J].新疆农业科学,2011,48(1):45-47.
[5] 萨茹丽,刘敏.蓝刺头花絮水提物的降血糖作用研究[J].西北农林科技大学学报:自然科学版,2015(6):47-51.
[6] 王华,曹婧,翟丽娟,等.猕猴桃果肉提取物抗氧化活性研究[J].华北农学报,2013,28(2):144-149.
[7] 乌仁张嘎.沙葱挥发油的提取,成分鉴定及其体外抑菌效果的研究[D].呼和浩特:内蒙古农业大学,2011.
[8] 许颖,李德超,孙剑.金银花醇提液对根管粪肠球菌生物膜作用体外研究[J].黑龙江医药科学,2010,33(6):49-50.
[9] 刘姚,欧阳克蕙,葛霞,等.植物多糖生物活性研究进展[J].江苏农业科学,2013,41(1):1-4.
[10] 张璃,李沙沙.植物多糖与化妆品的联系[J].辽宁中医药大学学报,2013,15(1):109-111.
[11] 周静华,左绍远.植物多糖生物学活性研究进展[J].大理学院学报:综合版,2012,11(6):35-37.
[12] 张志军,李淑芳,魏雪生,等.灵芝多糖体外抗氧化活性的研究[J].化学与生物工程,2011,28(3):63-65.
[13] 罗旭艳,黄建春,杨欣,等.杨桃根多糖体外抗氧化作用的研究[J].中国实验方剂学杂志,2011,17(4):111-114.
[14] 尚晓娅,张媛,白艳玲,等.绞股蓝多糖体外抗氧化活性研究[J].天然产物研究与开发,2013,25(4):450-454.
[15] 周紫燕,王坦,洪晓鹏.抗氧化天然活性多糖[J].日用化学品科学,2012,35(7):24-26,34.
[16] 凌洪锋,苏丹,曹洋.黄芪多糖抗氧化作用研究[J].医学理论与实践,2005,18(8):872-874.
[17] 张彩凤.多糖物质抗衰老作用的研究进展[J].生命科学仪器,2011,9(6):44-45.
[18] 王长云.多糖抗病毒作用[J].生物工程进展,2000(1):17-20.
[19] 张明.不同种属参多糖对免疫及抗菌作用的影响[J].中国兽医杂志,2010,46(1):54-56.
[20] He F,Yang Y,Yang G,et al.Studies on antibacterial activity and antibacterial mechanism of a novel polysaccharide from Streptomyces virginia H03[J].Food Control,2010,21(9):1257-1262.
[21] 张占军.薤白多糖的制备、性质、结构及其生物活性研究[D].南京:南京农业大学,2012.
[22] Buddle B M,Pulford H D,Ralston M.Protective effect of glucan against experimentally induced staphylococcal mastitis in ewes[J].Veterinary Microbiology,1988,16(1):67-76.

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