Optimization of Preparation Procedures for Exopolysaccharides from Lactobacillus casei LC2W

doi:10.7668/hbnxb.2008.S2.026

Abstract

Abstract: The parameters involved in preparation of exopolysaccharides (EPS) from Lactobacillus casei LC2W werestudied. Supernatant fermented MRS broth by Lb. casei LC2W containing EPS was firstly concentrated by ultrafiltration,then proteins in the retentate were eliminated by addition of trichlorocetic acid,and finally exopolysaccharides were pre2cipitated by alcohol. Results of single factor test and orthogonal experiments showed the optimized EPS preparation proce2dure was as the following:the supernantant of cultured MRS broth was firstly ultrafiltrated to 1/ 4 of original volume by us2ing MWCO of 10 kDa membrane,the proteins in the retentate was eliminated by TCA at an ultimate concentration of 6%( m/ V),and finnally 5 volumes of 95% ethanol alcohol of the retentate were added and the EPS was allowed to precipateat 4℃for 24 h.During this procedure,88135 %of the proteins was eliminated and the EPS recovery rate was reached to921.7 %.

Key words: Lactobacillus casei , Exopolysaccharide , Preparation

CLC Number:

TS24

LIU Cui-ping, YE Lei, WU Zheng-jun, WANG Yin-yu, GUO Ben-heng,. Optimization of Preparation Procedures for Exopolysaccharides from Lactobacillus casei LC2W[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 118-121. doi: 10.7668/hbnxb.2008.S2.026.

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