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An Efficient Method for Preparation of the Total RNA from the Root of Mangrove

FU Xiu-mei2, HAN Shu-mei2, ZHU Hong-lin2, ZHOU Xiu2, LI Xiao-jing2, WU hui2, XIE Jun2, CHEN Yin-hua1,2   

  1. 1 Key Laboratory for Tropical Biological Resources, Ministry of Education, Haikou 570228, China;
    2 College of Agronomy, Hainan University, Haikou 570228, China
  • Received:2009-10-02 Published:2009-12-31

Abstract: The mangrove is an important salt-tolerance plant. In this research, three method for isolation of total RNA from the root of mangrove were done. The improved method of CTAB was verified to be the most efficient method through the way of the electrophoretic patterns of nucleic acids and absorbanee at 230, 260 and 280 nm in a UV-Vis spectrophotometer.

Key words: Mangrove, Preparation of RNA, Method of CTAB

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Cite this article

FU Xiu-mei, HAN Shu-mei, ZHU Hong-lin, ZHOU Xiu, LI Xiao-jing, WU hui, XIE Jun, CHEN Yin-hua. An Efficient Method for Preparation of the Total RNA from the Root of Mangrove[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.004.

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