Construction of A Plant Expression Vector Targeting the Arabidopsis thaliana pre-miR399b Gene

doi:10.7668/hbnxb.2010.06.006

Abstract

Abstract: miR399 could regulate Pi homeostasis, consists of six members(miR399a-f)in Arabidopsis thaliana.Here we cloned pre-miR399b fragment with primers designed according to the stem—loop sequence.The fragement was inserted into the multiple cloning sites of the binary vector pJG045 with Ligation-Independent cloning.The vector was transformed into the Agrobacterium tumefaciens strain EHAl05.It may provide an excellent foundation for functional verification of miR399b in soybean and cultivating high Pi·-efficiency soybean by delivering pre—miR399b gene into soybean.

Key words: Arabidopsis thaliana, pre—miR399b, Vector construction

CLC Number:

Q943.2

SUN Dian-chen, SHA Ai-hua, SHAN Zhi-hui, CHEN Li-miao, ZHOU Xin-an. Construction of A Plant Expression Vector Targeting the Arabidopsis thaliana pre-miR399b Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 30-33. doi: 10.7668/hbnxb.2010.06.006.

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