Isolation of Poor Homologous Synapsis 1 Promoter and Construction of Its Expressing Vector in Arabidopsis tlucliaha

doi:10.7668/hbnxb.2009.01.022

Abstract

Abstract: Meiosis is crucial in sexual reproduction. It has been shown that poor homologous synapeis 1(phsl)ire-quired for homologous clmomosome pairing and for prevent synapsis between nonhomologous chromosomess. Promoter ofphsl was isolated by PCR from Arabidcpsas genome in order to study the copression of phsl,thereafter phsl and its promotor were cloned onto copression vector including green fluorescent protein and yellow fluorescent protein. At last, corerstructed vectors were transformed into agrobacterlum so that the vectors are to be transformed into Arabadcpsis.

Key words: Meiosis, Synapsis, phsl, Fluorescent protein, Arabadopsas

CLC Number:

Q943.2

ZHAO Jun-liang, WU Dong-tang, WANG Xiu-ying, LI Gai-zhen, WU Yi-lun. Isolation of Poor Homologous Synapsis 1 Promoter and Construction of Its Expressing Vector in Arabidopsis tlucliaha[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 97-102. doi: 10.7668/hbnxb.2009.01.022.

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