Maintenance of Meiotic Arrest in Goat Oocyte by Follicular Cells

doi:10.7668/hbnxb.2008.04.028

Abstract

Abstract: In this experiment,during the process of goat oocyte IVM,by adding different concentration cumulus cell and its surrounding granular cell,we studied the inhibitive effects and its mechanism of follicular cells on meiosis of oocytes. The IVM rate of oocyte was not being affected considerably after adding 10 ⁵ and 10⁶cell/ mL cumulus cells or surrounding granular cells,contrast to control group (p > 0.05),but were significantly higher than that of 5 × 10⁶cell/ mL(41.2 %),10⁷ cell/ mL(24.6 %)cumulus cells and 5 × 10⁶ cell/ mL(38.5 %),10⁷ cell/ mL(23.8 %) surrounding granu-lar cells(p < 0.05). In adding 5 × 10⁶,10⁷cell/ mL cumulus cells group and surrounding granular cells group,the cumu-lus could not expand.Nuclear Status Examination,the meiosis of oocytes was maintained in G V stage. Rp-cyclic adenosine monophosphothioate,a membrane permeable antagonist to cAMP,induced the oocyte IVM rate of 10⁷cell/ mL cumulus cells group (71.6 %)and surrounding granular cells group (71.3 %)which there was no significant difference among the control group (p > 0.05). during the process of oocyte IVM,adding some concentration cumulus cells and surrounding granular cells would maintain meiotic arrest by increasing the cAMP level of oocyte,make oocyte stay in the G V stage.

Key words: G oat, Follicular cell, Oocyte, Meiosis, Meiotic arrest

CLC Number:

S826

LIU Li-jie, QUAN Fu-sheng, LIU Jun, ZHANG Dong, LIU Gen-sheng, ZHANG Yong. Maintenance of Meiotic Arrest in Goat Oocyte by Follicular Cells[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 119-123. doi: 10.7668/hbnxb.2008.04.028.

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