Effect of Cumulus Cells on in vitro Maturation of Bovine Oocytes

doi:10.7668/hbnxb.2014.04.018

Abstract

Abstract: To improve the in vitro maturation rate of bovine oocytes,the present study was carried out to determine the effects of cumulus cell on in vitro maturation rate of cumulus-oocytes-complexs(COCS)and artificial denuded oocytes(DOs),and its pathway and strength was also investigated.The results showed that co-culture with discrete cumulus cells significantly improved the in vitro maturation rate of denudes oocytes(57.98±14.27 vs 35.53± 14.00,P<0.05),but had no effects on COCS(76.66±5.77 vs 66.76±9.46,P>0.05);Co-culture with surrounding cumulus cell did not significantly improve the in vitro maturation rate of denudes oocytes(35.83±18.32 vs 35.53±14.00,P>0.05).The analysis results showed that cumulus cell promoted the in vitro maturation of oocytes by the pathway of gap-junction,however discrete cumulus cells worked by the pathway of paracrine;Three or more layers cumulus improved the mean maturation ability of their surrounded oocytes by 85.34%,added discrete cumulus cells separately improved the mean maturation ability of COCs and DOs by 16.18% and 60.61%.

Key words: Cumulus cells, Oocytes, In vitro maturation, Bovine

CLC Number:

S823.3

HUANG Cheng-jun, GUO Lin-lin, SUN Shou-qiang, LIN Feng, LIU Hai-jun. Effect of Cumulus Cells on in vitro Maturation of Bovine Oocytes[J]. journal1, 2014, 29(4): 111-115. doi: 10.7668/hbnxb.2014.04.018.

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http://www.hbnxb.net/EN/Y2014/V29/I4/111

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