Abstract: The objective of the current study was to characterize the concentrations of immunoglobulins(IgA,IgG,IgM) and lactoferrin in different bovine milk. Sandwich enzyme}inked immunosorbent assay (ELISA) was used to quantify the immunoglobulins and lactoferrin(Lf) contents in Yak milk,Buffalo milk and Holstein cow milk samAles,and correlation analysis was completed. The results showed that immunoglobulins and lactoferrin in Yak milk,Buffalo milk and Holstein cow milk samples had a good linear relationsship between concentration and absorbance values,and the standard curve equation R was greater than 0.99. There were significant differences of IgA,IgG IgM,and Lf in different milk ( P<0.05).The contents of IgA,IgG,IgM and Lf of species showed remarkable individual differences. IgA and IgM in Holstein cow milk showed a negative correlation,while the others species had a positive correlation. The results indicated that the content of immunoglobulins and lactoferrin in the milk were influenced by genetic factors and had each characteristic. According to the correlation of immunoglobulins and lactoferrin, forecasting the content of immunoglobulins and lactoferrin within species may be possible.

Key words: Species, Bovine milk, Immunoglobulin, Lactoferrin, ELISA, Concentration, Correlation

CLC Number: 

• S823