Abstract: In this research,bovine oocytes were dealed with different concentration of CdCl2(0,0.8,1.6,2.4 g/mL),we detect the change of oocyte mature rate,Ca2+distribution,apoptosis rates,the mitochondrial membrane potential,Bcl-2 and Bax gene expression,this study lay the foundation for the further research that is the influence of heavy metal to female mammals germ cell development and cell apoptosis mechanisms.Bovine oocytes were treated with different concentration of CdCl2(0,0.8,1.6,2.4 μg/mL)for 22 h,we count the rate of maturity and apoptosis;The early apoptosis of bovine oocytes was detected by Annexin V-FITC;The change of Ca2+distribution and the mitochondrial membrane potential were respectively detected by Fluo-3/AM and Rh123;Using RT-PCR,we detected the relative expression of Bcl-2 and Bax.The mature rate and apoptosis rate of the bovine oocytes by 0.8 g/mL treatment group compared with control group was not significant(P0.05);1.6 μg/mL and 2.4 μg/mL treatment group compared with control group were not significant(P0.05);with the increase of concentration of CdCl2,Ca2+ fluorescence intensity in cytoplasm was increasing,the mitochondrial membrane potential gradually declined,Bcl-2 and Bax Relative ratio was descending.The induction of oocytes apoptos is by CdCl2 is dose effect,which can restrain oocytes mature and promote cell apoptosis.

Key words: CdCl2, Bovine oocytes, In vitro maturation, Apoptosis

CLC Number: 

• S823