Abstract: To lay a foundation for further explore of the possible function and mechanism of miR-181a-5p in geese granulosa cell. Based on various network database, we predicted the potential targets and associated signaling pathways of miR-181a-5p through bioinformatics methods. Then the expression of miR-181a-5p and its targets in different stages granular layer were detected by qPCR.The results showed that miR-181a-5p was highly conserved in vertebrates. The GO and KEGG analysis revealed that miR-181a-5p mainly involved in the regulation of cell proliferation, differentiation and apoptosis. Most of target genes were mainly enriched in FOXO, MAPK, and PI3K-Akt signaling pathways. According to the interaction network of target genes, 10 core target genes (VEGFA, MAPK1, FOS, KRAS, HRAS, SIRT1, BCL2, ESR1, PTEN, CDKN1A) were screened out and most of these targets were associated with cell proliferation and apoptosis. The levels of miR-181a-5p was the highest in 2-4 mm follicles,but declined sharply in the 4-6 mm and showed an upward trend in the 4-6 mm, 8-10 mm, and F5 follicles.miR-181a-5p might regulate the SIRT1, BCL2, CDKN1B, PTEN, etc, then participate in FOXO, MAPK, PI3K-Akt signaling pathway to regulate the proliferation and apoptosis of granulosa cells.

Key words: miR-181a-5p, GO and KEGG analysis, Expression analysis, Granulosa cells, Proliferation, Apoptosis

CLC Number: 

• S835