Analysis of the Relationship Between NtCIPK Family Gene Expression and Potassium Content in Tobacco

doi:10.7668/hbnxb.2018.06.012

Abstract

Abstract: To explore the relationship between tobacco NtCIPK family gene expression and the potassium content of tobacco, and to provide reference for the molecular mechanism of potassium absorption in tobacco. For common tobacco varieties K326, Guiyan 5 and Yun 87 three different potassium concentrations (6.0, 1.0,0.2 mmol/L) were cultured in solution and their dry weight and potassium content were measured. At the same time, 11 genes of the NtCIPK family were analyzed for different potassium concentrations using qRT-PCR. Induced expression pattern. With the increased of potassium concentration in the culture medium, the dry weight and potassium content of the tested plants showed an increasing trend, and there were significant differences between treatments. Among them, Yun 87 is treated with different potassium concentrations (1.0, 0.2 mmol/L). Dry weight and potassium content were the highest. The expression of 11 genes in the NtCIPK family was induced by the change of potassium concentration, and showed up-regulated expression with the decreased of K⁺ concentration; only the expression of NtCIPK9 in K326 and NtCIPK24 in Guiyan 5 showed a downward trend.The results of path analysis showed that the expression of NtCIPK3 and NtCIPK5 was significantly positively correlated with the potassium content of K326 and Guiyan 5, respectively, at the concentration of 0.2 mmol/L K⁺, while NtCIPK23 was treated at the concentration of 6.0 mmol/L K⁺. The expression had a significant negative correlation with the potassium content per plant of Yun 87. The potassium content of tobacco was closely related to the expression of NtCIPK gene, and the enhanced expression of NtCIPK gene contributes to the absorption and accumulation of potassium by tobacco.

Key words: Tobacco, Potassium content, NtCIPK, Gene expression

CLC Number:

Q78
S572.03

YANG Shangyu, ZHUO Wei, CHEN Qian, JIANG Yao, TONG Zhu, LI Liqin, REN Xueliang, LU Liming. Analysis of the Relationship Between NtCIPK Family Gene Expression and Potassium Content in Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 88-94. doi: 10.7668/hbnxb.2018.06.012.

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