Studies on Goat Oocyte in vitro Maturation

doi:10.3321/j.issn:1000-7091.2006.05.020

Abstract

Abstract: The experiments had been carried out to develop an improved in vitro maturation system for goat oocytes by comparing the collecting methods of oocytes and the effects of FSH,LH and culture time on goat oocytes in vitro maturation.The result had showed that a greater number of useable oocyte was obtained by slicing method than by aspiration method.An addition of 10% FCS,1-2 mg/L 17β-E₂,10 mmol/L Hepes,0.12 mg/mL pyruvate sodium,0.1 mg/mL streptomycin,0.125 mg/mL penicillin,10 mg/L FSH,20-50 mg/L LH in the TCM199 medium had been effective to goat oocyte maturation in vitro,and to the extruding of the first polar body.The rate of maturation of goat oocyte in vitro cultured for 20,22,24 hours had been remarkably higher than those cultured for 18,26,30 hours(P<0.05).

Key words: Goat, Oocyte, in vitro Maturation

CLC Number:

S827.3

LI Ying, JIN Shi-ying, ZHANG Shu-jun, ZHOU Huan-min. Studies on Goat Oocyte in vitro Maturation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(5): 83-86. doi: 10.3321/j.issn:1000-7091.2006.05.020.

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