Apoptosis in Immune Organs of Pigs Induced by Porcine Circovirus Type 2-like Agent P1

doi:10.7668/hbnxb.2008.S2.019

Abstract

Abstract: The research is to study the apoptosis of pigs induced by porcine circovirus type 2??like agent P1. Theapoptosis was detected by TUNEL assay in immune organ samples( tonsil, spleen, superficial inguinal lymph node) of pigsinfected by P1 molecular clone.The results showed that the apoptosis??specific signal was detected in immune organ sam??ples( tonsil, spleen, superficial inguinal lymph node) at 21 and 35 d after P1 infection.The result suggests that P1 appearsto be related to immunosuppression resulted in apoptosis of lymphocyte.

Key words: Porcine circovirus type 2, P1, Apoptosis, TUNEL

CLC Number:

S852.65

WEN Li-bin, HE Kong-wang, YANG Han-chun, WANG Yu-ran, DONG Mei-xiang. Apoptosis in Immune Organs of Pigs Induced by Porcine Circovirus Type 2-like Agent P1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 88-91. doi: 10.7668/hbnxb.2008.S2.019.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.S2.019

http://www.hbnxb.net/EN/Y2008/V23/IS2/88

References

[1] Allan G,Meehan B,Todd D,et al.Novel porcine circovirus from pigs with wasting disease syndrome[J].Vet Rec,1998,142:467-468.
[2] Ellis J,Hassard L,Clark E,et al.Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome[J].Can Vet J,1998,39:44-51.
[3] West K H,Bystrom J M,Wojnarowiez C,et al.Myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2[J].J Vet Diagn Invest,1999,11:530-532.
[4] Rosell C,Segal(e)s J,Ramos-Vara J A,et al.Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome[J].Vet Rec,2000,146(2):40-43.
[5] Opriessnig T,McKeown N E,Harmon K L,et al.Porcine circovirus type 2 infection decreases the efficacy of a modified live porcine reproductive and respiratory syndrome virus vaccine[J].Clin Vaccine Immunol,2006,13(8):923-929.
[6] 杨汉春.猪免疫抑制性疾病的流行特点与控制对策[J].中国畜牧兽医,2004,31(5):41-43.
[7] Rosell C,Segal(e)s J,Plana-Dur(a)n J,et al.Pathological,immunohistochemical,and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs[J].J Comp Pathol,1999,120(1):59-78.
[8] Bolin S R,Stoffregen W C,Nayar G P,et al.Postweaning multisystemic wasting syndrome induced after experimental inoculation of cesarean-derived,colostrum-deprived piglets with type 2 porcine circovlrus[J].J Vet Diagn Invest,2001,13:185-194.
[9] Shibahara T,Sato K,Ishikawa Y,et al.Porcine circovirus induces B lymphocyte depletion in pigs with wasting disease syndrome[J].J Vet Med Sci,2000,62(11):1125-1131.
[10] Kiupel M,Stevenson G W,Choi J,et al.Viral replication and lesions in BALB/c mice experimentally inoculated with porcine circovirus isolated from a pig with postweaning multisystemie wasting disease[J].Vet Pathol,2001,38(1):74-82.
[11] Resendes A R,Maj(o) N,Segal(e)s J,et al.Apoptosis in lymphoid organs of pigs naturally infected by porcine circovirus type 2[J].J Gen Virol,2004,85:2837-2844.
[12] Mandrioli L,Sarli G,Panarese S,et al.Apoptosis and proliferative activity in lymph node reaction in postweaning multisystemic wasting syndrome(PMWS)[J].Vet Immunol Immunop,2004,97:25-37.
[13] 温立斌,何孔旺,杨汉春.类猪圆环病毒2型因子P1与P2株全长基因组DNA分子克隆的构建[J].江苏农业学报,2007,23:579-582.
[14] 温立斌,何孔旺,杨汉春.P1因子分子克隆的体外感染性分析[J].畜牧兽医学报,2008,39:941-944.
[15] Kerr J F R,Wyllie A H,Currie A B.Apoptosis:a basic biological phenomenon with wide ranging implications in tissue kinetics[J].Brit J Cancer,1972,26(4):239-257.
[16] Ezoe H,Fatt R B,Mak S.Degradation of intracellular DNA in KB cells infected with cyt mutants of human adenovirus type 12[J].J Virol,1981,40:20-27.
[17] Sirinarumitr T,Zhang Y,Kluge J P,et al.A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo[J].J Gen Virol,1998,79:2989-2995.
[18] Sur J H,Doster A R,Osorio F A.Apoptosis induced in vivo during acute inoculation by porcine reproductive and respiratory syndrome virus[J].Vet Pathol,1998,35(6):506-514.
[19] 张秀珊,曹洪战,张丽青,等.河北地区猪圆环病毒二型ORF2基因片段的克隆与原核表达[J].华北农学报,2008,23(4):51-54.

Please choose a citation manager

Content to export