Cloning and Expression Analysis of Aquaporin TaTIP1-4DL Gene in Wheat

doi:10.7668/hbnxb.201751747

Abstract

Abstract: The TIPs family is a type of membrane intrinsic protein located on the tonoplast, which is responsible for regulating cell turgor and responds to external stress. To further explore the expression pattern and biological function of TIPs gene in wheat, the TaTIP1-4DL gene was amplified from flag leaves of wheat by homologous cloning. The coding region was 771 bp in length and encoded 257 amino acids. Bioinformatics analysis showed that the protein had a molecular weight of 26.3 ku and a theoretical isoelectric point of 6.82, belonging to a hydrophobic stability protein. The tertiary structure was a conservative hourglass structure containing six long alpha helices and two short alpha helices, corresponding to the classic model of aquaporin. The amino acid alignment and phylogenetic tree analysis showed that the TaTIP1-4DL protein of wheat was closely related to the homology of Aegilops Linn.and Brachypodium distachyou(L.) Beauv.The promoter cis-acting element contained the response element related to stress. Tissue-specific expression analysis showed that TaTIP1-4DL gene was expressed in different tissues and organs of wheat, and the highest expression level was in leaves and the least in stems. The stress expression analysis showed that the expression level of TaTIP1-4DL gene in leaves and seeds was induced in different degrees by drought, abscisic acid and high salt, and the expression level was generally up-regulated and then decreased. Among them, drought induced the highest level of the gene in leaves. After 6 hours of drought treatment, the expression of the gene in leaves reached 14 times of that before stress.

Key words: Wheat, TaTIP1-4DL gene, Gene cloning, Expression analysis

CLC Number:

S512.1+1

ZHANG Shan, GUO Qiping, LIU Yuanyuan, DANG Renmei, WEN Shanshan. Cloning and Expression Analysis of Aquaporin TaTIP1-4DL Gene in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 1-7. doi: 10.7668/hbnxb.201751747.

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