Apoptosis in Detached Corn Root Cap Cells Treated by HMC-toxin Under Fluorescent Microscope

doi:10.7668/hbnxb.2009.S2.030

Abstract

Abstract: Apoptosis in detached corn root cap cells under fluorescent microscope was studied. A pair of homokaryon(CB37 and B37)inbred lines in maize was tested and the detached corn root cap cells might be using acridine orange staining. Apoptosis was found under fluorescence microscope treated by fihrated culture fluid of HMC toxin. The sensitivity of HMC toxin to C cytoplasm was greater than N cytoplasm, so that the quantity of the apoptosis was great more in the detached corn root cap cells with C cytoplasm than with N cytoplasm. Besides, apoptosis was not found in the CK. These indicated that special disease response occurred in the course of apoptosis in detached corn root cap cells of C cytoplasm maize led by HMC.

Key words: Helminthosporium maydis race C (HMC), Filtrated culture fluid of toxin, Corn root cap cell, Apoptosis

CLC Number:

S513.01

MA Chun-hong, ZHENG Qiu-ling, DONG Wen-qi, LI Xiu-li, ZHANG Hong-xin, LI Yun-chao, SHANG Chuang, FAN Wei-wei, JIA Yin-suo. Apoptosis in Detached Corn Root Cap Cells Treated by HMC-toxin Under Fluorescent Microscope[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.030.

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References

[1] Miler R J, Koeppe D E.Southern corn leaf blight:Succeptible and resistant mitoehondria[J].Science, 1971, 173:67-69.

[2] Bhullar B S, Daly J M, Rehfeld D W.Inhibition of Dark CO₂ fixation and photosynthesis in leaf discs of corn susceptible to the host-specific toxin produced by Helminthosporium maydis race T[J].Plant Physiol, 1975, 56:1-7.

[3] 王立安, 赵俊霞, 马春红, 等.玉米根冠脱落细胞中微丝分布的荧光显微观察[J].实验生物学报, 2003, 36(2):149-154.

[4] 王立安, 马春红, 赵俊霞, 等.HMC毒素对C型不育玉米根冠细胞原生质体及微丝分布的影响[J].植物病理学报, 2003, 33(3):225-228.

[5] Smedgard-peterson V.Nelson R R.The production of a host-specific pathotoxin by Coehliobolus heterostrophus[J].Canadian Journal of Botany, 1969, 47:951-957.

[6] Lim S M, Hooker A L, Smith D R.Use of Helminthosporium maydis race T pathotoxin to determine disease reaction of germinating corn seed I J].Agronomy Journal, 1971, 63:712-713.

[7] Hawes M C.Technique for using isolated corn root cap cells in a simple quantitative assay for the pathotoxin produced by Helminthosporium maydis race T[J].Phytopathology, 1983, 73 (8):1184-1187.

[8] 张利辉, 邢继红, 董金皋.玉米大斑病菌2号小种毒素的生物测定与组分分析[J].河北农业大学学报, 2001, 24(1):42-45.

[9] 郑秋玲, 顾梦贤.稻瘟病毒素对水稻根冠细胞的影响[J].华北农学报, 1991, 6(2):108-111.

[10] 郭兰凯, 刘俊芳, 马春红, 等.HMC对雄花不育及正常细胞质玉米根冠活细胞的影响[J].中国农业科学, 1989, 22(3):94-95.

[11] 戴广辉, 刘俊芳, 李延增, 等.棉花黄萎病抗病性鉴定新方法探讨[J].华北农学报, 1989, 4(4):92-97.

[12] 郭兰凯, 刘俊芳, 马春红, 等.HMC毒素对雄花不育及正常细胞质玉米根冠细胞的影响[J].作物学报, 1991.17:54-57.

[13] 刘克明, 吴全安, 刘俊芳, 等.玉米小斑病菌三个生理小种生物学特性比较的初步研究[J].华北农学报, 1989, 4(2):74-78.

[14] 周建华, 丛国正, 高闪电, 等.细胞凋亡的研究进展[J], 生物技术通讯, 2008, 19(2):274-276.

[15] Wang H, Li J, Bostock R M, et al.Apoptosis:A functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development[J].Plant Cell, 1996, 8 (3):375-391.

[16] 宁顺斌, 宋运淳, 王玲.低温胁迫诱导玉米根尖细胞凋亡的形态和生化证据[J].植物生理学报, 2000, 6(3):189-194.

[17] Jonathan D, Walton.Host-selective toxins:agents of compatibility[Jl.Plant Cell, 1996, 8:1723-1733.

[18] Scheffer R P.Role of toxins in evolution and ecology of plant pathogenic fungi[J].Experienti, 1991, 47:804-811.

[19] 崔洋, 刘克明, 魏建昆.玉米小斑病菌C小种毒素的分离、纯化及其植物病理反应[J].植物病理学报, 1991, 21(3):187-191.

[20] 范尉尉, 陈霞, 马春红, 等.大丽轮枝菌粗毒素在棉花根冠细胞生物学测定方法上的研究[J].华北农学报, 2006, 21(1):55-58.

[21] 潘耀谦, 于艳, 夏志平.检测细胞凋亡的常用方法[J].动物医学进展, 2001, 22(2):32-36.

[22] 金钢, 叶建仁.植物细胞程序性死亡(PCD)分析测试方法的发展[J].山西农业大学学报, 2006, 26(2):113-118.

[23] 鄂征.组织培养技术[M].第2版.北京:人民卫生出版社, 1986:202-204.

[24] Meehan F, Murphy H C.Differential phytoxicity of metabolie by-products of Helminthosporiurn victoriae[J].Science 1947, 106:270-271.

[25] Yoder O C.Toxins in pathogenesis[J].Annual Review of Phytopathology, 1980, 81103-129.

[26] 王敬文, 薛应龙.植物苯丙氨酸解氨酶的研究Ⅲ:玉米小斑病菌T小种和大斑病菌毒素对苯丙氨酸解氨酶(PAL)的刺激作用[J].植物生理学报, 1982, 8(3):237-244.

[27] 马春红, 商阁, 贾银锁, 等.HMC毒素培养滤液诱导C103玉米抗小斑病的研究[J].中国农业科学:英文版, 2006, 5(9):678-684.

[28] 马春红, 翟彩霞, 王立安, 等.玉米小斑病菌T小种培养滤液对玉米抗病性的诱导[J].华中农业大学学报:自然科学版, 2008, 27(5):590-596.

[29] 商闯, 贾银锁, 马春红, 等.HMC毒素培养滤液对专化寄主玉米叶片诱导抗病性及相关酶的影响[J].中国农业科学, 2008, 41(12):4307-4313.

[30] 商闯, 马春红, 贾银锁, 等.低浓度玉米小斑病菌C小种毒素培养滤液诱导寄主抗性的研究[J].华北农学报, 2008, 23(2):198-204.

[31] 商闯, 贾银锁, 侯立白, 等.玉米离体根冠细胞凋亡的荧光显微观察(简报)[J].分子细胞生物学报, 2008, 41(4):329-334.

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