Abstract: The objective is to obtain expression profile of Chinese Simmental cattle ucp1,ucp2,ucp3 genes,to reveals the relationship between the ucp1,ucp2,ucp3 gene and energy metabolism,to explore the expression rule of key gene of fat deposition,and to screen the candidate gene affecting fat deposition.Total RNA were extracted from fifteen tissues(liver,lymph,intercostals muscle,shoulder muscle,spleen,kidney,rib eye muscle,lung,heart,gut fat,greater omental fat,testicle,small intestine,pancreas and backfat)of twenty animals from Chinese Simmental cattle.The primers of ucp1,ucp2,ucp3 genes were designed and synthesized,and the data were normalized using the β-actin gene as internal control.The specific expression of ucp1,ucp2,ucp3 genes in different tissues were investigated by real-time quantitative PCR.There were different degree expression of ucp1,ucp2,ucp3 genes mRNA in the tissues and organs of testicle,small intestine,heart,pancreas and so on.The expression of ucp1 was highest in the backfat,middle in the pancreas,small intestine,testicle,greater omental fat,gut fat,and heart,and lowest in the other tissues.The highest level expression of ucp 2 was detected in Testicle,and there were also higher expression of ucp2 in the lung,liver,and backfat than in other tissues.The highest level expression of ucp3 was in rib eye muscle,Intercostal muscle,and shoulder muscle,middle in the lung,and the lowest in other tissues.Correlation analyses showed that the expression level of ucp3 gene in rib eye had no significant relation with intramuscular fat and backfat thickness,but had positive relation to eye lean area(r= 0.788).It is concluded that the expression level of ucp1,ucp2,ucp3 genes had tissue specific might be related to the various functions on different tissues of cattle.The ucp3 gene can be used as a candidate gene related to carcass traits.

Key words: Uncoupling proteins(UCPs), Tissue and organs, Gene expression, Carcass traits

CLC Number: 

• S823