Effects of NaCl Stress on Nitrogen Metabolism and Related Gene Expression of Rice with Different Resistance

doi:10.7668/hbnxb.20190507

Abstract

Abstract: In order to study the changes of nitrogen metabolism of rice varieties with different resistant under salt stress, the resistant rice Dongdao 4 and the sensitive variety Nipponbare were used as materials, and changes of nitrogen content, nitrogen-related enzymes and gene expression in rice roots and leaves under NaCl stress were investigated. The results showed that the nitrate nitrogen content, nitric acid reductase (NR) activity and glutamine synthetase(GS), NADH-dependent glutamate synthase (NADH-GOGAT) enzyme activity of rice seedings treated by 0.3% NaCl in roots and leaves reduced. While the ammonium nitrogen content, the activity of ferrodoxin-dependent glutamate synthase (Fd-GOGAT), glutamate dehydrogenase(GDH) and aspartic acid aminotransferase(AST) increased. The nitrogen content and nitrogen metabolic enzyme activity of Dongdao 4 were more stable than Nipponbare, and the changes of roots and leaves were consistent. Detection of genes expression associated with nitrogen transport and metabolism found that: The expression of nitrate transporter protein genes OsNRT1; 1, OsNRT1; 5, OsNTR1; 8, OsNRT2; 1, ammonium transporter protein genes OsAMT1; 1, OsAMT1; 2, glutamine synthase gene OsGS1; 2, transaminase gene OsAS had high expression after NaCl stress and significantly increased in the high expression tissue. Glutamate synthase gene OsFd-GOGAT increased significantly in the leaves of both cultivars and decreased slightly in the roots. Gutamate synthase gene OsNADH-GOGAT and glutamate dehydrogenase gene OsGDH were elevated significantly in the roots of Nipponbare, but not significantly in leaves and also in the Dongdao 4. The expression of OsNR1 was significantly reduced in leaves of the two varieties, but no difference in roots.On the whole the expression of OsNRTs and the genes related to nitrogen metabolism in Dongdao 4 were higher than that of Nipponbare, but OsAMTs in Nipponbare increase more than Dongdao 4. The study showed that Dongdao 4 could maintain more stable nitrogen metabolic than Nipponbare, and this might be an important mechanism for the salt tolerance of rice resistant variety.

Key words: Rice, Salt stress, Nitrogen metabolism, Gene expression

CLC Number:

S511.03
Q78

YANG Decui, XU Qing, ZHAO Fanggui, LIU Mian, LIU Xin. Effects of NaCl Stress on Nitrogen Metabolism and Related Gene Expression of Rice with Different Resistance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 187-195. doi: 10.7668/hbnxb.20190507.

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