ISSR Analysis of Genetic Diversity in Asparagus

doi:10.7668/hbnxb.2014.04.020

Abstract

Abstract: The genetic diversity of 43 asparagus cultivars collected from 5 countries was analyzed by ISSR method in order to provide more data reference for asparagus breeding.The results show that a total of 159 bands were amplified by PCR with 18 primers,among which 121 bands were polymorphic.The percentage of polymorphism was 76.10%.For these asparagus,the observed number of alleles was 2,the effective number of alleles was 1.444 3,the Nei's gene diversity was 0.265 4,and the Shannon's information index was 0.411 4.Calculated using the formula,the average polymorphism index content(PIC) was 0.669,ranged from 0.317 to 0.840.The genomic average genetic richness was 5.17.This indicates that the genetic diversity in the tested materials with high performance.The genetic similarity coefficient was analyzed,and a tree diagram was constructed by NTSYS 2.10e software.UPGMA cluster analysis showed that these cultivars could be categorized eight groups according to coefficient 0.77.The cultivars JK104 from Jiangxi,Pacific Peak from Spain and 082 from Shandong were respectively identified as different single group.In other group,the genetic similarity between the cultivars from American and Jiangxi,American and Holland,Holland and Beijing were relatively high.

Key words: Asparagus, ISSR, Cluster analysis, Genetic diversity

CLC Number:

S644.6

QI Xian-hui, LI Gai-zhen, WU Dong-tang. ISSR Analysis of Genetic Diversity in Asparagus[J]. journal1, 2014, 29(4): 121-125. doi: 10.7668/hbnxb.2014.04.020.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.04.020

http://www.hbnxb.net/EN/Y2014/V29/I4/121

References

[1] 张玉霞,张立军,王艳树,等.秋季低温对芦笋渗透调节物质和抗氧化酶活性的影响[J].华北农学报,2006,21(6):63-67.
[2] 丁茂发.山西芦笋业的困境和发展对策[J].山西农业科学,2007,35(12):16-18.
[3] 刘金荣,徐淑霞,周青,等.芦笋生物学特征及栽培技术[J].河南农业科学,2003(9):59.
[4] 宋德庆,张劲,薛忠,等.我国芦笋产业发展现状与对策[J].广东农业科学,2011(21):187-190.
[5] 代真真,江程记,杨峰,等.24份芦笋种质的ISSR分析[J].热带农业科学,2012,32(5):35-40.
[6] 马利平,郝变青,乔雄梧,等.山西省芦笋病害现状及无公害生产技术[J].山西农业科学,2009,37(1):69-73.
[7] Hollingsworth W O,Christie C B,Nichols M A,et al.Detection of variation among and within asparagus hybrids using random amplified DNA(RAPD) markers[J].New Zealand Journal of Crop&Horticultural Science,1998,26(1):1-9.
[8] Khandka D K,Nejidat A.Polymorphism and DNA markers for asparagus cultivars identified by random amplified polymorphic DNA[J].Euphytica,1996,87:39-44.
[9] Jaag T,Langsdorf A,Snowdon R J,et al.Random amplified polymorphic DNA variation among German and Dutch asparagus cultivars[J].Plant Breeding,2006,117(3):290-292.
[10] 李可峰.石刁柏(Asparagus officinalis L.)种质资源遗传多样性分析[D].济南:山东师范大学,2004.
[11] 李霞,刁家连,李书华,等.芦笋种质资源遗传多样性的RAPD分析[J].植物遗传资源学报,2005,6(3):310-314.
[12] 韩莹琰,范双喜,付婕.芦笋AFLP技术体系的建立与优化[C]//中国园艺学会第七届青年学术讨论会论文集.山东:中国园艺学会,2006:292-295.
[13] 盛文涛,周劲松,汤咏萍,等.芦笋SRAP反应体系优化及引物筛选[J].分子植物育种,2010,8(3):612-618.
[14] 李霞,于继庆,李书华,等.利用正交设计优化芦笋SRAP反应体系[J].农学学报,2011,1(3):5-9.
[15] Maria Sica,Graziella Gamba,Stefania Montieri,etal.ISSR markers show differentiation among Italian populations of Asparagus acutifolius L[J].BMC Genetics,2005,6:7.
[16] 盛文涛,周劲松,汤泳萍,等.芦笋基因组DNA提取与ISSR反应体系的优化[J].江西农业学报,2010,22(9):38-41.
[17] 周延清.DNA分子标记技术在植物研究中的应用[M].北京:化学工业出版社,2005:1,143.
[18] 崔金腾,陈冰嬬,石英尧,等.供试品种的遗传多样性与籼型水稻优良恢复系回交导入改良[J].分子植物育种,2008,6(1):25-31.
[19] 郝晨阳,王兰芬,张学勇,等.我国育成小麦品种的遗传多样性演变[J].中国科学C辑:生命科学,2005,35(5):408-415.

Please choose a citation manager

Content to export