Abstract: Genetic diversity between the different Chinese Jujube cultivars was researched by Random Amplified Polymorphic DNA( RAPD) molecular marker technology. The optimized RAPD analysis system of genetic molecular marker on PCR amplification technology must be built. Various concentration of dNTPs, random primer, Taq DNA polymerase, Mg²⁺buffer and DNA template quality and concentrationwas set up. Conclusion of the optimized contents of 20 μ L RAPD reaction system was as follows: 2 μ L 10 ×Taq DNA polymerase Buffer, 2. 0 mmol/L Mg ²⁺, 200 μ mol/L dNTPs, 0. 2 μ mol/L primer,0. 06 U/μ L Taq DNA polymerase and 1. 5 ng/μ L DNA template. The optimized PCR cycle program was as follows: 94℃4 min,94℃30 s,36℃40 s,72℃ 1 min,50 cycles and at final 72℃8 min .

Key words: Chinese Jujube, RAPD, Optimized analysis system

CLC Number: 

• S665.1