Effects of Duration on Oocyte Cytoplasm Maturation in vitro with CGs Staining

doi:10.7668/hbnxb.2011.03.020

Abstract

Abstract: In order to evaluate the effect of duration on oocyte cytoplasm maturation,the oocytes matured 24,28,32,36 and 43 h were fixed in 3.7% paraformaldehyde and stained in FITC-LCA and PI,and the distribution of cortical granules(CGs) was observed to evaluate the cytoplasmic maturation.CGs were found throughout the oocyte cytoplasm.The CGFD didn't appear in oocytes of domestic cat.There were more CGs located in the cortex of the oocytes which were matured 32 h than the other groups.CGs decreased in oocytes matured 36 h and 43 h.The resultes showed that oocytes matured 32 h got ready to be sitimulated,and after 32 h the oocytes were degraded.Therefore,32 h was the best maturation duration of domestic cat oocytes IVM.

Key words: Domestic cat, Oocyte, Cortical granules

CLC Number:

S88.293

WANG Xin-zhuang, QIN Jia-chen, REN Wei-qing, HE Jun-dan, LU Jing-yu. Effects of Duration on Oocyte Cytoplasm Maturation in vitro with CGs Staining[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 95-99. doi: 10.7668/hbnxb.2011.03.020.

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References

[1] Gulyas B J.Cortical granules of mammalian eggs[J].Int Rev Cytol,1980,63:357-392.

[2] Damiani P,Fissore R A,Cibelli J B,et al.Evaluation of developmental competence,nuclear and ooplasmic maturation of caff oocytes[J].Mol Reprod Dev,1996,45,521-534.

[3] 马红.激素对牛卵母细胞体外成熟的影响[J].华北农学报,2009,24(增刊):338-339.

[4] 刘海军,刘灵,刘玉堂,等.受体卵母细胞来源和供体细胞类型对山羊体细胞核移植融合率的影响[J].华北农学报,2008,23(1):41-44.

[5] 王星,罗光彬,刘永,等.生长激素和胰岛素对猪卵母细胞体外成熟及卵裂的影响[J].河南农业科学,2009(1):100-103.

[6] 李晓霞,曹平华,邓星,等.不同浓度的牛血清及牛血清白蛋白对水牛卵母细胞体外成熟的影响[J].河南农业科学,2008(7):108-111.

[7] Miyara F,Aubriot F X,Glissant A,et al.Multiparameter analysis of human oocytes at metaphase Ⅱ stage after IVF failure in non-male infertility[J].Hum Reprod,2003,18:1494-1503.

[8] Zamboni L.Ultrastructure of mammalian oocytes and ova[J].Biol Reprod,1970,2:44-63.

[9] Hoodbhoy T,Talbot P.Mammalian cortical granules:contents,fate,and function[J].Mol Reprod Dev,1994,39:439-448.

[10] Ducibella T,Buetow J.Competence to undergo normal,fertilizationinduced cortical activation develops after metaphase Ⅰ of meiosis in mouse oocytes[J].Der Biol,1994,165:95-104.

[11] Schuel H.Function of egg cortical granules[D].New York,USA:Metz CB and Monroy A,1985.

[12] Gwatkin R B L.Fertilization mechanisms in man and mammals[M].New York:Plenum,1997.

[13] Yanagimachi R.Mammalian fertilization[D].New York:Knobil E and Neill J D,1994.

[14] DucibellaT,Kurasawa S,Rangarajan S,et al.Precocious loss of cortical granules during mouse oocyte meiotic maturation and correlation with an egg-induced modification of the Zona pellucida[J].Dev Biol,1990,137:46-55.

[15] Ducibella T,Duffy P,Reindollar R,et al.Changes in the distribution of mouse oocyte cortical granules and ability to undergo the cortical reaction during gonadotropin-stimulated meiotic maturation and aging in vivo[J].Biol Reprod,1990,43:870-876.

[16] Liu M,Sims D,Calarco P,et al.Biochemical heterogeneity,migration,and pre-fertilization release of mouse oocyte cortical granules[J].Reprod Biol Endocrino,2003,1(1):77.

[17] Byers A P,Barone M A,Donoghue A M,et al.Matration domestic cat oocytes does not express a cortical granule-free domain[J].Biol Reprod,1992,47:709-715.

[18] Mikkelsen A L,Lindenbegrg S.Morphology of in vitro matured oocytes:Impact on fertility potential and embryo quality[J].Hum RePord,2001,16:1714-1718.

[19] Khatir H,Lonegran P,Caorlan C,et al.Prepubertal bovine oocyte:a negative model for studying oocyte development competence[J].Mol Reprod Dev,1996,45:231-239.

[20] Naito K,Fukuda Y,Toyoda Y.Effects of porcine follicular fluid on male pornucleus of maturation in porcine oocytes matured in vitor[J].Gamet Research,1988,21:289-295.

[21] 李荣凤,薛晓先,刘哲,等.牛卵母细胞皮层颗粒荧光染色法质成熟鉴定[J].畜牧兽医学报,2000,31(3):203-210.

[22] De Felici M,Siracusa G.Spontaneous hardening of the zona pellucida of mouse oocytes during in vitro culture[J].Gamete Res,1982,6:107-113.

[23] Zhang X,Rutledge J,Amrstrong D T.Studies on zona hardening in rat oocytes that are matured in vitor in a serum-free medium[J].Mol ReProd Dev,1991,28:292-296.

[24] Downs S M,Schoreder A C,Eppig J J.Seurm maintains the fertilizability of mouse oocytes matured in vitor by preventing the hardening of the Zona pellucida[J].Gamete Res,1986,15:115-122.

[25] Fujii Y,Yoshioka T,Saskai J.Fetal calf serum increased the Zona pellucida penetrability of rat oocytes matured in vitor[J].Acta Med Okayama,1990,44:203-208.

[26] Dell' Aquila M E,Cho Y S,Minoial P,et al.Effects of follicular fluid supplementation of in-vitro maturation medium on the fertilization and development of equine oocytes after in-vitor fertilization or intracytoplasmic sperm injection[J].Hum Reprod,1997,12:2766-2772.

[27] Peter K,Gregoyr S K,Riehadr M S.Modifications of the mouse Zona pellucida during oocyte muatration and egg activation:Effects of new born calf serum and fetuin[J].Biol Reprod,1991:783-787.

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