Molecular Cloning and Sequence Analysis of Oryctolagus cuniculus CD147

doi:10.7668/hbnxb.2009.01.021

Abstract

Abstract: A pair of cloning primers were designed based on the in silico sequence information and were used toclone and analysis the Oryctolagus cundculus CD147. Total RNA was extracted from the duode num mucosal tissue of O-ryctolagus cuniculus and mRNA sequence was amplified by -The positive clone was identified and sequenced. Theopenreading frame of Oryctolagus cundculus CDl47consists of 810 by and encodes 270 amino acids.The signal peptide ofdeduced protein sequence includes 1-16 amino acids.The predicted peptide shared 62. 5%,65. 9%,59. 5%and66. 3%homology with those of Oryctolagus cundculus,Bostaurus,Homo sapdens,Rattus norvegdcus and Sus scrofa. Thetertiary structure prediction presented that CD147 contains two extracellularlike cbmains. The Oryctolagus cundculusCD147 gene has been submitted to GenBank(Accession EU650668).

Key words: Oryctolagus cuniculus, CD147, Molecular cloning, Sequence analysis, Structure prediction

CLC Number:

S852.4

JIA Hai-li, HAN Li-qiang, YANG Guo-yu, ZHONG Kai, GUO Yu-jie, TAI Yu-lei, LIU Tao, LI Ping, WANG Yan-ling. Molecular Cloning and Sequence Analysis of Oryctolagus cuniculus CD147[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 93-96. doi: 10.7668/hbnxb.2009.01.021.

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http://www.hbnxb.net/EN/Y2009/V24/I1/93

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