GFP-Tagging and Colonization Observation of the Endophytic Fungus DZJ07 from Eucommia ulmoides

doi:10.7668/hbnxb.2017.06.019

Abstract

Abstract: Colletotrichum sp. DZJ07, a biological control strain isolated from the Eucommia ulmoides, shows potential for control of wheat sharp eyespot.To elucidate the ecological adaptability of DZJ07 of wheat, the green fluorescent protein gene(gfp)was inserted into the DZJ07 genome by Agrobacterium -mediated transformation method, and a GFP-tagged DZJ07-6 strain was constructed. Colony shape, growth rate, antagonistic effect and efficacy for biological control of wheat sharp eyespot were analyzed with DZJ07 and the GFP-tagged strain DZJ07-6.No significant differences were obtained for any of the characteristics tested between the two strains. Then colonization ability of the GFP-tagged strain DZJ07-6 on wheat was assessed under greenhouse conditions. And observing by fluorescence microscope showed that the marked strain was able to colonize in the root and stem of wheat, and the population of DZJ07-6 in wheat rhizosphere, root and stem after 34 days were 2.72×10⁶, 0.62×10⁴ and 2.26×10³ cfu/g respectively. The results afforded scientific basis for its colonization and the further research on bio-controlling mechanism.

Key words: Endophytic fungi DZJ07, Green fluorescent protein, Wheat sharp eyespot, Colonization

CLC Number:

S432.4+4

DING Ting, GU Shuangyue, SU Bo, WANG Qi, CHEN Xiaojie. GFP-Tagging and Colonization Observation of the Endophytic Fungus DZJ07 from Eucommia ulmoides[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 126-133. doi: 10.7668/hbnxb.2017.06.019.

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http://www.hbnxb.net/EN/Y2017/V32/I6/126

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