Abstract: This work would help for wheat breeding that we could get some low phosphorus tolerance germplasm resources via the methods of the genetic transformation of AeBiPAP1 into wheat cultivals.In this study, PAP1 gene was cloned from Aegilop bicornis with designed primers according the sequence of PAP1 genes from wheat and slen der falsebrome based on homologous cloning method and named AeBiPAP1.The cloned AeBiPAP1 gene was 1.124 kb in size and encoded 335 amino acids, with molecular weight of 38.131 kDa and isoelectric point 5.77.Sequence alignment of AeBiPAP1 with PAP1 of wheat showed that there were 35 single nucleotide polymorphisms, 22 locus transitions and 13 locus transversion and AeBiPAP1 had high homology to PAP1 of wheat with the identities of 95.52%.Amino acid sequence alignment showed that 15 deduced amino acids of AeBiPAP1 were different from PAP1 of wheat.Bioinformatics analysis of AeBiPAP1 gene encoding protein found that the protein has a signal peptide but have not the across-membrane structure and the AeBiPAP1 coding protein with tertiary structure prediction found that the encoded protein contains the metal ions center predicted it belongs to the metal protein.Besides, its possibility of localization in the plasma membrane is higher than being secreted to outside of the cell, the phylogenic tree results showed that PAP1 gene from Aegilop bicornis shared high similarities to the previously reported PAP1 genes from other plants, such as Triticum aestivum, Brachypodium distachyon, and Setaria italica, among them, AeBiPAP1 gene shared the highest similarities to PAP1 gene from Triticum aestivum.

Key words: Aegilop bicornis, Purple acid phosphotase, Low phosphorus tolerance gene

CLC Number: 

• Q78

• Q943.2